Page 47 - Application Notebook - Solution for Food Safety

Application No. C161
News

OH O OH O O
Compound name Chlortetracycline OH
Accurate mass 479.1216 [M+H]+ NH 2
Formula C22H23ClN2O8
CAS 57-62-5 OH
H H
Cl H O CH 3 N
H 3 C CH 3
MRM Spectrum Mode
11 MRM’s acquired for
chlortetracycline at 10pg/uL in egg.
1:479.10>444.00 (+) CE: -23V 7:479.10>300.80(+) CE: -45V
2:479.10>461.95 (+) CE: -35V 8:479.10>287.90(+) CE: -53V
3:479.10>154.00 (+) CE: -34V 9:479.10>274.95(+) CE: -44V
4:479.10>98.05(+) CE: -45V 10:479.10>370.95(+) CE: -31V
5:479.10>260.05(+) CE: -60V 11:479.10>285.85(+) CE: -56V
6:479.10>303.05(+) CE: -37V
MRM Spectrum mode
Higher specificity
Higher reporting confidence
Library searchable fragment data.
The number of precursor-fragment ion transitions monitored is limited only by the structural chemistry of the molecule.
6.50 6.75 7.00 7.25 7.50 min Typically more than 10 precursor-fragment ion transitions were monitored for each veterinary drug.

Utilization of MRM Spectrum Mode (Chlortetracycline)

Fig. 2 shows the MRM reference spectrum for As the collision energy was optimized for each
chlortetracycline with assigned fragment structures. fragment ion to generate a product ion spectrum, the
The MRM Spectrum mode is a measurement mode library spectrum is highly specific and selective.
which combines MRM with the generation of a product
ion spectrum. The product ion spectrum can be used
for compound identification by searching a library.

m/z 154.00 m/z 274.95 m/z 444.00 m/z 461.95
[Accurate mass 154.05042] [Accurate mass 275.04749] [Accurate mass 444.08501] [Accurate mass 462.09557]
Formula C7H8NO3 (+4H) Formula C15H12O3Cl (+4H) Formula C22H19NO7Cl (+2H) Formula C22H21NO8Cl
+ OH O OH O O
+ OH 2 O OH OH O OH O O OH
OH O OH C +
C +
OH
OH Cl H O CH 3 N
Cl CH 3 Cl H O CH 3 N 444.00 H 3 C CH 3
N H 3 C CH 3
H 3 C CH 3

m/z 303.05
[Accurate mass 303.04241] 461.95
Formula C16H12O4Cl +(4H)
m/z 98.05
[Accurate mass 98.06059]
Formula C5H8NO (+4H) OH O OH
C
H 3 +
OH 154.00
m/z 260.05 +
N [Accurate mass 260.02402] CH 2
H C
3 CH 3 Formula C14H9O3Cl• (+4H) Cl H O CH 3
O OH
98.05
C +
CH 2 260.05
274.95 303.05
CH 3 370.95
Cl H O CH 3 287.90
100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 m/z

MRM Reference Spectrum with Assigned Fragment Structures (Chlortetracycline)