Page 43 - Application Notebook - Solution for Food Safety

Application No.C99
News

n MRM Analysis of Matrix Standards
Fig. 3 shows the MRM chromatogram of the matrix the compounds using only standard solution. Although
standard solution consisting of the sample solution with there were several compounds for which the lower limit
added standard solution (data obtained using pork of quantitation was different in the standard solution
extract solution). Table 1 shows the lower limits of than the matrix-added solution, rather than attributing
quantitation for the standard solution without added this to matrix effects, it is thought to be caused by
matrix and with added matrix, respectively. In a crude elevated background due to ions derived from
extract obtained by acetonitrile extraction alone, contaminating components (Refer to Fig. 5).
sensitivity was comparable to that obtained for most of

NJO

Fig. 3 MRM Chromatograms of 89 Veterinary Drugs (10 µg/L pork extract solution with added standard solution)

Table 1 LOQs of Veterinary Drugs in Neat Standards and Matrix Standards and Calibration
Range of Veterinary Drugs in Matrix Standards
Std. Solution Matrix-Added Std. Solution Std. Solution Matrix-Added Std. Solution
Min. Conc. Min. Conc. Max. Conc. Min. Conc. Min. Conc. Max. Conc.
Gentamicin 0.5 1 50 Sulfachloropyridazine 0.02 0.02 20
Sulfanilamide 1 1 50 Sulfadimethoxine 0.02 0.02 10
Levamisole 0.05 0.05 50 Tylosin 0.05 0.05 50
Lincomycin 0.01 0.01 10 Sulfamethoxazole 0.02 0.1 10
5-Propylsulfonyl-1-benzimidazole-2- 0.05 0.05 10 Sulfaethoxypyridazine 0.02 0.02 10
amine Tiamulin 0.01 0.01 50
Diaveridine 0.01 0.01 10 Florfenicol 0.5 10 50
Trimethoprim 0.02 0.02 20 2Acetylamino 5nitrothiazole 0.05 0.05 50
Marbofloxacin 0.01 0.01 50 Sulfatroxazole 0.01 0.01 5
Sulfisomidine 0.02 0.02 20 Leucomycin 0.01 0.01 50
Norfloxacin 0.5 0.5 50 Sulfisoxazole 0.01 0.05 50
Ormetoprim 0.02 0.02 10 Oxolinic acid 0.01 0.1 50
Thiabendazole 0.01 0.01 10 Chloramphenicol 0.5 1 50
Ciprofloxacin 0.05 0.5 10 Clorsulon 0.5 1 50
Neospiramycin I 0.01 0.05 10 Sulfabenzamide 0.01 0.01 10
Danofloxacin 0.1 0.1 10 Ethopabate 0.01 0.01 10
Enrofloxacin 0.05 0.1 50 Sulfadoxine 0.02 0.02 20
Oxytetracycline 0.01 0.1 50 Sulfaquinoxaline 0.02 0.02 10
Xylazine 0.01 0.01 10 Prednisolone 0.1 0.05 20
Orbifloxacin 0.05 0.05 50 Ofloxacin 0.5 0.5 50
Sulfacetamide 1 1 50 Flubendazole 0.01 0.01 50
Clenbuterol 0.01 0.01 10 Methylprednisolone 0.5 0.5 50
Tetracycline 0.05 0.01 50 Nalidixic acid 0.01 0.01 50
Spiramycin I 0.01 0.01 50 Dexamethasone 0.5 0.5 50
Sarafloxacin 0.5 0.5 50 Flumequine 0.01 0.01 50
Difloxacin 0.05 0.1 50 Benzylpenicillin 0.5 0.5 50
Sulfadiazine 0.02 0.1 20 Sulfanitran 0.2 0.2 50
Sulfathiazole 0.02 0.1 20 Sulfabromomethazine 0.01 0.01 50
Sulfapyridine 0.02 0.1 20 betaTrenbolone 0.02 0.1 50
Carbadox 0.05 0.05 10 Emamectin B1a 0.01 0.01 50
Pyrimethamine 0.02 0.02 20 alphaTrenbolone 0.02 0.1 50
Sulfamerazine 0.02 0.02 20 Piromidic acid 0.01 0.05 50
Chlortetracycline 0.1 0.1 50 Zeranol 1 0.1 50
Tilmicosin 0.1 0.1 50 Ketoprofen 0.01 0.05 50
Thiamphenicol 1 1 50 Testosterone 0.01 0.05 10
Sulfadimidine 0.02 0.02 20 Famphur 0.05 0.05 50
Sulfametoxydiazine 0.01 0.02 10 Fenobucarb (BPMC) 0.01 0.01 50
Sulfamethoxypyridazine 0.02 0.02 20 Clostebol 0.05 0.05 50
Sulfisozole 0.01 0.01 50 Dichlofenac 0.01 0.01 50
Trichlorfon (DEP) 0.05 0.05 50 Melengestrol Acetate 0.05 0.05 50
Sulfamonomethoxine 0.02 0.02 20 Temephos (Abate) 0.01 0.5 50
Furazolidone 1 1 50 Allethrin 0.1 1 50
Difurazone 0.05 0.05 50 Closantel 0.01 0.01 10
Erythromycin A 0.01 0.01 50 Monensin 0.01 0.01 10
Cefazolin 0.5 0.5 50
(Unit: µg/L)