Page 44 - Application Notebook - Solution for Food Safety

Application No.C99
News

Clenbuterol Sulfamerazine
Area Area
3000 22:277.10>203.05(+) 1750000 2000 32:265.10>92.10(+) 2500000
2000 Pork 1500000 1500 Pork 2000000
1000
1000 +0.01 ppb +0.01 ppb
1250000 500
0 0 1500000
2.50 2.75 3.00 3.25 3.50 3.75 1000000 3.25 3.50 3.75 4.00 4.25 4.50
3000 22:277.10>203.05(+) 750000 2000 32:265.10>92.10(+) 1000000
2000 Neat STD 500000 0.01-10 ppb 1500 Neat STD 500000 0.01-10 ppb
1000
1000 0.01 ppb 250000 0.01 ppb
500
0 0 0 0
2.50 2.75 3.00 3.25 3.50 3.75 0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.010.0 3.25 3.50 3.75 4.00 4.25 4.50 0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0
Conc. Conc.
β-trenbolone Area Orbifloxacin Area
2000000
2000 69:271.20>199.10(+) 3000000 20:396.00>352.20(+) 1750000
1500 750
1000 Pork 2500000 500 Pork 1500000
500 +0.1 ppb 250 +0.05 ppb 1250000
0 2000000 0
7.25 7.50 7.75 8.00 8.25 8.50 2.50 2.75 3.00 3.25 3.50 3.75 1000000
1500000
2000 69:271.20>199.10(+) 20:396.00>352.20(+) 750000
1500 1000000 750 500000
1000 Neat STD 0.1-50 ppb 500 Neat STD 0.05-50 ppb
500 0.1 ppb 500000 250 0.05 ppb 250000
0 0 0 0
50.0
7.25 7.50 7.75 8.00 8.25 8.50 0.0 10.0 20.0 30.0 40.0 50.0 2.50 2.75 3.00 3.25 3.50 3.75 0.0 10.0 20.0 30.0 40.0 Conc.
Conc.
Fig. 4 MRM Chromatograms in the Vicinity of the LOQ and Calibration Curves of Typical Compounds

n Recoveries of Veterinary Drugs in Crude Extracts
from Livestock and Fishery Products
(Matrix Effect Verification)
We examined whether or not the matrix affected which the rates of recovery were determined. The
measurement of actual samples. This time, four types of results indicated that 90 % of the compounds were
food product samples were used, including shrimp, recovered at rates of 70 to 120 % and measurement
chicken meat, pork, and salmon. Standard solution was was accomplished without any adverse matrix effects
added to the acetonitrile extraction solution of each of even though the crude extract solution was subjected
these to obtain a final concentration of 10 µg/L, after only to acetonitrile extraction.

Shrimp Chicken Meat
150 150

(%) 100 100
Recovery 50 50

0 0
Peak elution order

Pork Salmon
150 150

100 100

50 50

0 0

Fig. 5 Recoveries of Veterinary Drugs in Each of the Matrices