Page 16 - Application Notebook - Solution for Food Development

LAAN-A-LM-E104

Application Liquid Chromatography Mass Spectrometry

News Simultaneous Analysis of 16 Sweeteners Using

Triple Quadrupole LC/MS/MS [LCMS-8050]
No.C133

Artificial sweeteners such as aspartame, sucralose, and Q Standard Mixture Analysis
acesulfame potassium fall under the category of MRM analysis was performed on 16 sweeteners
designated additives according to Japan's Food using the analytical conditions shown in Table 1.
Sanitation Act, and prescribed standards are in place Chromatograms of each compound near their lower
for their use in some foods and quantities. limit of quantitation are shown in Fig. 1, with
Cyclamate and other artificial sweeteners used in calibration curve ranges and correlation coefficients
some regions outside Japan are included among shown in Table 2. Results that met an accuracy of
undesignated additives in Japan, and inspection is 100 % ±20 % and area repeatability (%RSD) of
required in specific imported foods. within 20 % were used for calibration point. Good
Consequently, quantitation for large numbers of linearity was obtained for all compounds, with
sweeteners, including not only permitted in Japan but correlation coefficients of 0.997 or higher.
also undesignated, are needed.
Application News C121 described the simultaneous
analysis of nine artificial sweeteners including both
designated and undesignated additives using an
LCMS-8040 triple quadrupole LC/MS/MS system. In
this article, we introduce an example of simultaneous
analysis of 16 sweeteners using an LCMS-8050.

Table 1 Analytical Conditions
Column : Unison UK-C18 Injection Volume : 1 μL
(150 mm L. × 3.0 mm I.D., 3.0 μm) Probe Voltage : 4.0 kV (ESI-positive mode) /
Mobile Phases : A 5 mmol/L Ammonium formate - Water -3.0 kV (ESI-negative mode)
: B 5 mmol/L Ammonium formate - Methanol Nebulizing Gas Flow : 3 L/min
Gradient : B.Conc. 0 % (0.0-2.0 min) Heating Gas Flow : 10 L/min
ˠ 70 % (4.5 min) ˠ 90 % (8.0-12.0 min) Interface Temperature : 300 °C
ˠ 0 % (12.01-15.0 min) DL Temperature : 150 °C
Flowrate : 0.4 mL/min Block Heater Temperature : 250 °C
Column Temperature : 40 °C Drying Gas Flow : 10 L/min

ʢ×100ʣ ʢ×1,000ʣ ʢ×1,000ʣ ʢ×100ʣ
0.5 ng/mL 0.05 ng/mL 0.5 ng/mL 0.5 ng/mL
2.5 1.0 1.0 2.5

0.0 0.0 0.0
0.1
6.0 6.5 6.5 7.0 6.5 7.0 8.0 8.5
4VDSBMPTF %VMDJO "MJUBNF 3FCBVEJPTJEF "
ʢ×100ʣ ʢ×100ʣ ʢ×100ʣ ʢ×100ʣ
5.0
0.5 ng/mL 0.1 ng/mL 0.5 ng/mL 1 ng/mL
2.5 1.0
2.5 1.0

0.0 0.0 0.0 0.0
8.0 8.5 5.0 5.5 5.5 6.0 6.0 6.5
4UFWJPTJEF "DFTVMGBNF QPUBTTJVN 4BDDIBSJO $ZDMBNBUF

Fig. 1-1 Chromatograms of 16 Sweeteners