Page 15 - Application Notebook - Solution for Food Development

Application No.C121
News

100 Acesulfame potassium 175 Saccharin Cyclamate 400 Sucralose Aspartame
0.05 ng/mL 1 ng/mL 700 1 ng/mL 0.5 ng/mL 400 0.5 ng/mL
150
600
75
125 300
500 300
100 400
50 200
75 300 200
50
25 200 100
25 100 100
0 0 0 0
5.0 5.5 5.5 6.0 6.0 6.5 6.0 6.5 6.5 7.0
Dulcin 750 Neotame 2500 Rebaudioside A 2500 Stevioside
1000 0.05 ng/mL 0.05 ng/mL 5 ng/mL 5 ng/mL
2000
2000
750 500
1500 1500
500 1000 1000
250
250 500 500
0 0 0
0
6.5 7.0 7.5 8.0 8.0 8.5 8.0 8.5
Fig. 2 Chromatograms of Nine Sweeteners at Around LLOQ
Table 2 Linearity of Nine Sweeteners

Compound Name Polarity Transition Retention Time (min) Calibration Curve Range (ng/mL) Correlation
Coefficient
Acesulfame potassium − 162.00 > 82.10 5.228 0.05 − 100 0.997
Saccharin − 182.00 > 42.00 5.561 1 − 100 0.999
Cyclamate − 178.00 > 80.00 6.057 1 − 100 0.998
Sucralose + 413.90 > 199.00 6.370 0.5 − 500 0.999
Aspartame − 293.10 > 261.10 6.543 0.5 − 1000 0.999
Dulcin + 181.20 > 108.10 6.712 0.05 − 10 0.999
Neotame + 379.10 > 172.20 7.898 0.05 − 1000 0.999
Rebaudioside A − 965.30 > 803.40 8.220 5 − 1000 0.999
Stevioside + 822.30 > 319.20 8.238 5 − 1000 0.999

n Recovery from Actual Samples
Seven sweeteners were added to foods (curry paste, which the recovery was calculated based on a 1000-
rice cake flavored with mugwort, and sponge cake) fold dilution of the solution after dialysis treatment,
pretreated by dialysis (Fig. 3), and the matrix effect was while the recovery of all other sweetener samples was
evaluated. The recovery of each added sweetener is calculated based on 100-fold dilution. The recovery was
shown in Table 3. Dulcin was the only sweetener for good with all samples, ranging from 85 to 125 %.

Table 3 Recovery of Seven Added Sweeteners
Recovery (%) 20 g sample
Added
Compound Name Concentration Curry Paste Flavored with Chocolate ˣ
Rice Cake
Mugwort Sponge Cake
Dialysis (24 hours)
Acesulfame potassium 100.8 94.2 93.7
ˣ
Saccharin 97.0 87.7 88.3
Solution after dialysis
Cyclamate 99.6 89.3 92.0
Sucralose 5 µg/mL 96.2 89.6 82.6 ˣ 100-fold or 1000-fold dilution
Aspartame 94.0 89.4 87.2 LC/MS/MS analysis
Dulcin 110.2 99.5 99.5
Neotame 122.5 106.9 110.0 Fig. 3 Workflow of Pretreatment

This Application News was prepared with the cooperation of Tokyo Food Sanitation Association, who provided samples and guidance.

First Edition: Jan. 2016

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