Page 20 - Application Notebook - Solution for Food Development
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LAAN-A-GC-E061
Application Gas Chromatography
News
Aroma Component Analysis of Japanese Sake by
GC-FID Using a Diacetyl Derivatizing Reagent
No. G296
The food industry widely employs evaluation using Diacetyl in Japanese Sake
analytical instruments for new product developments and Diacetyl is a compound which has a characteristic aroma
quality assurance. In the brewing industry, aroma and greatly affects the quality of fermented beverage and
component analysis is highly important due to the strong food products. Although the aroma of diacetyl is
effects on product quality. Regarding aroma components indispensible for dairy products which are made by lactic
in brewed Japanese sake, the conventional analysis acid fermentation such as cultured butter and some
method uses a gas chromatograph combined with a cheeses, it is considered undesirable for food and drinks
headspace sampler. By combining this method with a which are made by alcoholic fermentation such as
high-sensitivity detector, detection of aroma components alcoholic beverages. In particular, sake breweries consider
in low concentrations is possible. However, such a system the aroma to be one of the most undesirable odors and
is expensive and sample preparation calls for an therefore conduct quantitative evaluation at production.
experienced analyst.
In this article, we introduce an example analysis of aroma
components in Japanese sake with a simple GC system
configuration using a simplified pretreatment method
which employs a derivatizing reagent manufactured by
Shinwa Chemical Industries. Derivatization Reaction of Diacetyl Using
E. Kobayashi, T. Murata Shinwa DS-DA
Diacetyl reacts with o-phenylenediamine and turns into
Shinwa DS-DA (Diacetyl) 2,3-dimethyl quinoxaline (DMQX). Although FID
Diacetyl Derivatization Kit sensitivity for diacetyl is low because diacetyl has two
Shinwa Chemical Industries carbonyl groups, DMQX obtained by derivatization has a
quinoxaline structure and therefore the FID sensitivity
Shinwa DS-DA is a reagent kit for derivatizing diacetyl in
non-alcoholic beverages and alcoholic beverages for rises.
measurement by gas chromatography.
Derivatization Reaction of Diacetyl Using Shinwa DS-DA
Procedure of Diacetyl Derivatization Using
Shinwa DS-DA
Derivatization was performed using the Shinwa DS-DA
(Diacetyl) reagent kit on a sample (Japanese sake). The
Shinwa DS-DA (Diacetyl)
diacetyl contained in the sample reacts and turns into
DMQX. Methyl n-propyl quinoxaline (MPQX) was added as
an internal standard substance.
Procedure of Diacetyl Derivatization Using Shinwa DS-DA
