Page 24 - Application Notebook - Solution for Food Development

Application AD-0124Ph
News

Table 5: GC Analytical Conditions for SPB-20 capillary Analyte Conc., ppm Analyte Conc., ppm
column 1. Acetaldehyde 43 9. n-Butyl acetate 35
2. Acetone 43 10. Isobutanol 32
3. Ethyl acetate 27 11. Isoamyl acetate 29
Injection Temp. 150 C 4. Acetal 26 12. N-butanol 39
o
5. Methanol 32 13. Isoamyl alcohol 28
6. Isopropanol 29 13a. Active amyl alcohol -
o
o
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Column Temp. 35 C (5 min), 5 C/min ~100 C (1.0 min) 7. N-propyl acetate 40 14. 1-Pentanol -
8. N-propanol 29 15. Furfural 42
Injection Mode Split, 1:10
Carrier Gas He
Linear Velocity 30 cm/sec
Injection Volume 1 µL
o
Detector 250 C, FID
Analyte* Conc., ppm
1. Acetaldehyde 9
3. Ethyl acetate 6
5. Methanol 10
8. n-propanol 7
10. iso-butanol 23
12. n-butanol 17
13. isoamyl alcohol 47
13a. active amyl alcohol 24 Acetaldehyde &
methanol
Figure 5: GC analysis of 15 alcohol congeners on Supel-Q
plot capillary column
 Conclusions

This study shows that the separation of the 15 alcohol
* Tested only for eight standards congeners which are the major components found in
alcoholic beverages can be achieved using capillary
Figure 4: GC analysis of 15 alcohol congeners on SPB-20 columns. A capillary column is thus a possible alternative
capillary column
to replace the conventional packed column for alcoholic
beverage analysis. However, the study also reveals that
The Supel-Q plot capillary column contains a porous none of a single packed (Carbowax 20M) or a capillary
sorbent made from copolymer of ethylbenzene and column can fully separate all the 15 alcohol congeners. As
divinylbenzene. The ethanol matrix interfered with the peak an option, the use of two different capillary columns may
of isopropanol for all capillary columns, except this one. provide a better solution, which can be accomplished on
This was the only column tested that was able to resolve the Shimadzu GC-2010, a GC system designed for dual-
isopropanol from the ethanol matrix (see Figure 5). column analyses of a same sample with two FID detectors
However, while this column separates the most number of simultaneously.
peaks, significantly smaller and broader peaks with slight
tailing was observed. This can be attributed to the column  References
inherently high adsorption characteristic.
[1] AOAC Official Methods 972.10. Alcohols (Higher) and Ethyl
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Acetate in Distilled Liquors, 2000, 17 ed.
Table 6: GC Analytical Conditions for Supel-Q Plot [2] AOAC Official Methods 972.11. Methanol in Distilled Liquors,
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Capillary column 2000, 17 ed, AOAC International.
[3] Official Journal of the European Communities L333/36.
Determination of Volatile Substances and Methanol of Spirit
o
Injection Temp. 200 C Drinks.
o
o
o
o
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50 C, 10 C/min ~150 C, 5 C/min ~210 C, [4] Restek Technical Guide. Analyzing Alcoholic Beverages by
Column Temp. o o
40 C/min ~250 C (5 min) Gas Chromatography. www.restekcorp.com
[5] Supelco Application Note 164. The Analysis of Alcoholic
Injection Mode Split, 1:10 Beverages on a 30m x 0.25mm ID x 1.0um SPB-20 Capillary
Carrier Gas He Column. www.sigmaaldrich.com
Linear Velocity 30 cm/sec [6] Supelco Bulletin 790C. Improved Resolution of Alcoholic
Beverage Components by Packed Column GC.
Injection Volume 1 µL
o
Detector 270 C, FID www.sigmaaldrich.com
Disclaimer: The Shimadzu GC-2010 system and the data in this
Application News are intended for Research Use Only (RUO). Not for use
in diagnostic procedures.

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