Page 21 - Application Notebook - Solution for Food Development

Application No. G296
News

„ Instrument Configuration and Analysis „ Analysis of Diacetyl Derivatives Obtained Using
Conditions Shinwa DS-DA
Table 1 lists the configuration of the instrument used for After derivatizing diacetyl, the peak from DMQX was
analysis and the analysis conditions. detected in the vicinity of a retention time of 9.6 min
(Fig. 4).
Table 1 Instrument Configuration and Analysis Conditions The dotted line in Fig. 5 indicates the calibration curve of a
Gas chromatograph : GC-2010 Plus 15 % ethanol solution which was prepared in accordance
Autosampler : AOC-20i with the composition of Japanese sake and then added
Sample injection port : SPL
Column : ULBON HR-52 diacetyl. The solid line is the calibration curve of a
(25 m × 0.25 mm I.D. df = 0.25 mm) commercially available sake which was added diacetyl.
Detector : Flame ionization detector (FID) From this we were able to determine that the diacetyl
Injection volume : 2 μL content in this commercially available sake is 125 ppb
Carrier gas : He, 30 cm/sec
Split ratio : 5:1 (μg/L).
Injection port temperature : 200 °C
Detector temperature : 280 °C
Column temperature : 100 °C (2 min) - 10 °C/min -
270 °C (5 min)

Diacetyl
derivative
DMQX

0 5.0 10.0 15.0 20.0 min
Chromatogram of Japanese Sake Sample After Diacetyl Derivatization

1
Solid line: Commercially available sake
Peak area ratio [DMQX / internal standard substance]
(contains diacetyl) with diacetyl added y = 0.0016x + 0.2065
Dotted line: Dummy sake R² = 0.9999
0.8 (contains no diacetyl) with diacetyl added

0.6
y = 0.0016x + 0.0056
R² = 0.9997
0.4

0.2

0
0 50 100 150 200 250 300 350 400 450 500
Diacetyl concentration / ppb
Calibration Curve of Diacetyl Derivative Sample
First Edition: Oct. 2017
For Research Use Only. Not for use in diagnostic procedure.
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