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Application No. C155
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„ Estimate of phospholipids by SimLipid „ Phospholipids in four tissue samples
Based on the PIS and NLS analysis results shown in Figure 3 shows the peak intensities “Total Abundance”
Figure 1, we conducted a database search using of phospholipids confirmed in all tissue samples
SimLipid software to estimate the phospholipids of among the PLs estimated in each tissue as a result of
each detected peak. First the data acquired by LCMS- the database search. From Figure 3, we can see the
8060 was read directly by SimLipid software and then phospholipids characteristic to each tissue. For
the peak intensity detected by PIS and NLS analysis example, PC(32:0) is known as the main component of
were calculated for each sample. We conducted a pulmonary surfactant which has the function of
database search on the basis of a tolerance, decreasing the surface tension of the alveoli, and
presence/absence of adduct ion and selection of PC(32:1) is also contained a lot. It is also known that
product ion (fatty acids or head groups) for the set there are many phosphatidylcholines including
mass value. In SimLipid software, thousands of palmitic acid (16:0) and oleic acid (18:1) in the cerebral
phospholipids are registered in the database and the cortex of the brain. Furthermore, we can see that
peaks detected under the preset conditions are many phospholipids are remarkably contained in the
compared with the database. Figure 2 shows an liver tissue in this result.
example of database search of lung tissue analysis
results. Here information about each phospholipid
class and the total number of carbon chain and By the database search using SimLipid software as
double bonds of fatty acids for the corresponding shown here, it enables to estimate a kind of
peak, are estimated as a result of database search. phospholipid contained in the sample from enormous
The integrated intensity of the corresponding peak phospholipids.
“Total Abundance” is displayed in Figure 2 (n=4).

Example of database search by SimLipid (Lung tissue)

7.E+08 4.E+06
6.E+08 3.E+06
5.E+08 3.E+06
4.E+08 2.E+06

3.E+08 2.E+06
2.E+08 1.E+06
1.E+08 5.E+05
0.E+00 0.E+00
PC(32:0) PC(32:1) PC(34:1) PC(36:2) PC(36:4) PC(38:4) SM(40:1) SM(40:3) SM(42:3) PI(38:3) PI(38:4) PS(36:4)

Brain Spleen Lung Liver Brain Spleen Lung Liver

“Total Abundance” of phospholipids in four tissue samples

First Edition: Apr. 2017
For Research Use Only. Not for use in diagnostic procedure.
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