Page 11 - Pharmaceutical Solution for Pharma Analysis

LC/MS/MS MRM Library for Phospholipid Profiling
For LabSolutions Version 5

Step 1 Step 2

Simultaneous Analysis Using the Peak Identification
Phospholipid Classification Method
The phospholipid peak detected by the
The phospholipid classification method is capable of phospholipid classification method is identified.
comprehensive analysis of the main phospholipids in The structural information on the detected
biological samples. It determines the phospholipid phospholipid at this point is the phospholipid
class based on the main characteristic head groups of class (PC, PE, PG, PI, PS, SM), and the total
phospholipids. Its analysis targets are phospholipids carbon number and number of double bonds
that include C14 to C22 fatty acids. in its constituent fatty acids. In the next step,
the fatty acid composition determination
method is created.
Step 3
(x1,000,000)
PC(36:1) 788.6>184.1 (+) 1.50
MRM Event-Link Editor 1.25
PC(36:1) 788.6>184.1 (+) Editing the Fatty Acid Composition 1.00
PC(14:0/22:1) 832.6>227.2 (−), 337.3 (−) Determination Method 0.75
PC(14:1/22:0) 832.6>225.2 (−), 339.3 (−) Software allows a fatty acid composition determination 0.50
PC(16:0/20:1) 832.6>255.2 (−), 309.3 (−) method to be edited from 867 MRM transitions for the 0.25
PC(16:1/20:0) 832.6>253.2 (−), 311.3 (−) phospholipid peak detected during first analysis.
PC(18:0/18:1) 832.6>283.3 (−), 281.3 (−) 0.00
7.5 10.0 12.5 15.0 17.5 20.0

Step 4 Step 5
(×10,000,000)
Simultaneous Analysis by Fatty Acid 1.50 PC(36:1) Data Analysis
1.25
Composition Determination Method 1.00
0.75 Based on the analysis results, it was determined that the sample contains
Edited in MRM Event-Link Editor, the fatty acid 0.50 PC (16:0/20:1) and PC (18:0/18:1), as shown in the figures below.
composition determination method is used to 0.25
0.00
perform a second analysis on the same sample. 16.0 17.0
Phospholipid profiling can be performed based on
the results of the first and second analysis. (×10) (×10) (×10,000) (×10) (×100,000)
PC(14:0/22:1) PC(14:1/22:0) 3.5 PC(16:0/20:1) PC(16:1/20:0) 4.0 PC(18:0/18:1)
(x1,000,000) 2.5 2.5 3.0 2.5
2.5 2.5 3.0
2.0
0.0 0.0 0.0
2.0 1.5 2.0
-2.5 -2.5 1.0 -2.5 1.0
0.5
1.5 0.0 0.0
-5.0 -5.0 -5.0
16.0 17.0 15.0 16.0 17.0 18.0 16.0 17.0 18.0 16.0 17.0 16.0 17.0
1.0
0.5
0.0
7.5 10.0 12.5 15.0 17.5 20.0

For Research Use Only. Not for use in diagnostic procedure.
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First Edition: June 2016, Printed in Japan