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C146-E314











            LC/MS/MS MRM Library for


            Phospholipid Profiling
            Phospholipid Profiling

            For LabSolutions Version 5























                                                                                                          LCMS-8060



            This MRM library includes two methods: one for phospholipid classification by comprehensive analysis of the main phospholipids in biological samples,
            and one for fatty acid composition determination created using analytical results obtained with the classification method. The library targets
            phospholipids containing C14 to C22 fatty acids, and includes MRM transitions for up to 867 components.
            This library enables performing phospholipid profiling by conducting an initial analysis with a phospholipid classification method. This is followed by
            creating a method for fatty acid composition determination based on the phospholipid peak detected in the first analysis, and subsequently using this
            method to perform a second analysis to determine fatty acid composition.


            Target Phospholipids                                MRM Transition Composition
            The phospholipids registered in the MRM library have fatty acid   Focusing on the characteristic phospholipid head group, the library
            compositions with the carbon number and double bond combinations   includes a method for phospholipid classification and a method for fatty
            shown in the table below. The phospholipid targets of the library are   acid composition determination (for fatty acid compositions of the given
            phosphatidylcholines (PC) with a lyso-group, phosphatidylethanolamines   combinations) that make use of these MRM transitions.
            (PE), phosphatidylglycerol (PG), phosphatidylinositol (PI),   The figure below shows each MRM transition required to identify PC
            phosphatidylserines (PS), and sphingomyelins (SM).  (18:1/16:0). The phospholipid can be inferred by combining the analytical
                                                                results obtained from these MRM transitions.


                                                                  MRM transition to determine   MRM transition to determine
                                Number of Double Bonds
                                                                  fatty acid composition    phospholipid classification
                      C14:0  C14:1
                      C16:0  C16:1
              Carbon
              Number  C18:0  C18:1  C18:2  C18:3
                                                                                                        +
                      C20:0  C20:1  C20:2  C20:3  C20:4  C20:5
                      C22:0  C22:1  C22:6
                                                                                    PC (18:1/16:0)
   5   6   7   8   9   10   11   12   13   14   15