Page 10 - Pharmaceutical Solution for Pharma Analysis
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C146-E314
LC/MS/MS MRM Library for
Phospholipid Profiling
Phospholipid Profiling
For LabSolutions Version 5
LCMS-8060
This MRM library includes two methods: one for phospholipid classification by comprehensive analysis of the main phospholipids in biological samples,
and one for fatty acid composition determination created using analytical results obtained with the classification method. The library targets
phospholipids containing C14 to C22 fatty acids, and includes MRM transitions for up to 867 components.
This library enables performing phospholipid profiling by conducting an initial analysis with a phospholipid classification method. This is followed by
creating a method for fatty acid composition determination based on the phospholipid peak detected in the first analysis, and subsequently using this
method to perform a second analysis to determine fatty acid composition.
Target Phospholipids MRM Transition Composition
The phospholipids registered in the MRM library have fatty acid Focusing on the characteristic phospholipid head group, the library
compositions with the carbon number and double bond combinations includes a method for phospholipid classification and a method for fatty
shown in the table below. The phospholipid targets of the library are acid composition determination (for fatty acid compositions of the given
phosphatidylcholines (PC) with a lyso-group, phosphatidylethanolamines combinations) that make use of these MRM transitions.
(PE), phosphatidylglycerol (PG), phosphatidylinositol (PI), The figure below shows each MRM transition required to identify PC
phosphatidylserines (PS), and sphingomyelins (SM). (18:1/16:0). The phospholipid can be inferred by combining the analytical
results obtained from these MRM transitions.
MRM transition to determine MRM transition to determine
Number of Double Bonds
fatty acid composition phospholipid classification
C14:0 C14:1
C16:0 C16:1
Carbon
Number C18:0 C18:1 C18:2 C18:3
+
C20:0 C20:1 C20:2 C20:3 C20:4 C20:5
C22:0 C22:1 C22:6
PC (18:1/16:0)