Page 62 - Shimadu Consumables Catalog

UHPLC/HPLC Columns

Shim-pack GIS HILIC

Ideal for the Separation of Highly Polar Basic Compounds
Shim-pack GIS HILIC is designed for Hydrophilic Interaction Bonded Phase Diol Groups
Liquid Chromatography (HILIC). It is chemically bonded with Particle Size 3 µm, 5 µm
a diol group, which provides excellent peak shape for basic Pore Size 10 nm
and neutral polar compounds. Surface Area 450 m /g
2
In addition, HILIC is a variation of normal phase mode. Carbon Loading 20 %
It is capable of using organic solvents mixed with water End-capping -
as mobile phase, while normal phase mode uses non- pH Range 2 to 7.5
aqueous organic solvents. In HILIC, the higher the organic USP Code L20
concentration in the solvents, the greater is the retention of
highly polar compounds.

Analysis Examples

Peaks
1. Allantoin
Conditions
Column : Shim-pack GIS HILIC (250 mmL. × 3.0 mmI.D., 5 µm)
(P/N: 227-30639-07)
Mobile Phase : A) 10 mmol/L Ammonium acetate
B) Acetonitrile
A/B = 5/95 (v/v)
Flow Rate : 0.4 mL/min
Col. Temp. : 40 ºC
Detection : UV 210 nm

Peaks (500 mg/L each)
1. Taurine
2. Inositol
Conditions
Column : Shim-pack GIS HILIC (150 mmL. × 3.0 mmI.D., 5 µm)
(P/N: 227-30639-06)
Mobile Phase : A) Water
B) Acetonitrile
A/B = 20/80 (v/v)
Flow Rate : 0.4 mL/min
Col. Temp. : 40 ºC
Detection : RID (35 ºC, positive)
Injection Vol. : 20 μL

Pg A-58