Page 64 - Shimadu Consumables Catalog

UHPLC/HPLC Columns

Shim-pack GIST NH2

Ideal for Sugar Analysis
New high-purity porous spherical silica chemically bonded Bonded Phase Aminopropyl Group
with the aminopropyl group ensures the superior stability of Particle Size 3 μm, 5 μm
Shim-pack GIST NH2. It is capable of the analysis of vitamin E Pore Size 10 nm
or simultaneous analysis of sugars that are hard to separate Surface Area 350 m /g
2
in reversed phase mode. In addition, due to being primarily Carbon Loading 7 %
amine-bound, Shim-pack GIST NH2 can analyze sugars with End-capping -
no separation of anomers, even under low-temperature pH Range 2 to 7.5
conditions. Furthermore, Shim-pack GIST NH2 delivers highly USP Code L8
reliable reproducibility and stability with accurate qualitative
and quantitative results.

Analysis Examples

Peaks
1. Fructose
2. Glucose
3. Sucrose
4. Maltose
Conditions
Column : Shim-pack GIST NH2 (250 mmL. × 4.6 mmI.D., 5 μm)
(P/N: 227-30302-08)
Mobile Phase : A) Water
B) Acetonitrile
A/B = 25/75, v/v
Flow Rate : 1.0 mL/min
Col. Temp. : 40 ºC
Detection : RID
Injection Vol. : 5 μL

Peaks (25 mg/L each)
1. α-Tocopherol
2. β-Tocopherol
3. γ-Tocopherol
4. δ-Tocopherol
Conditions
Column : Shim-pack GIST NH2 (250 mmL. × 4.6 mmI.D., 5 μm)
(P/N: 227-30302-08)
Mobile Phase : A) n-Hexane
B) Ethyl acetate
A/B = 70/30, v/v
Flow Rate : 1.0 mL/min
Col. Temp. : 40 ºC
Detection : UV 290 nm
Injection Vol. : 10 μL

Pg A-60