Page 68 - Shimadu Consumables Catalog

UHPLC/HPLC Columns

Shim-pack GIS CN

Suitable in Either Reversed Phase or Normal Phase Mode
Shim-pack GIS CN is capable of either normal phase or Bonded Phase Cyanopropryl Group
reversed phase analysis. Cyanopropryl groups bonded Particle Size 3 μm, 5 μm
to silica gel with high density increases the difference Pore Size 10 nm
recognition of hydrophilicity and the stability. Due to no Surface Area 450 m /g
2
end-capping, it is capable of analysis utilizing cyano group Carbon Loading 14 %
characteristics. End-capping -
In reversed phase mode, separation can be achieved for pH Range 2 to 7.5
those compounds that could not be separated on straight- USP Code L10
chain-alkyl columns, such as C18 or C8 bonded phases. When
using the column for reversed phase mode, fully equilibrate
the column before use.

Analysis Examples

γ Peaks
1. gamma-oryzanol
2. vanillin
Conditions
Column : Shim-pack GIS CN (150 mmL. × 4.6 mmI.D, 5 μm)
(P/N: 227-30263-06)
Mobile Phase : A) Hexane
B) 2-Propanol
C) Acetic acid
A/B/C = 94/5/1 (v/v/v)
Flow Rate : 1.0 mL/min
Col. Temp. : 30 ºC
Detection : UV 320 nm
Injection Vol. : 1 μL

Peaks
1. Asparagine • H 2 O (0.75 mg/mL)
2. Aspartic acid (0.75 mg/mL)
3. Fumaric acid (0.01 mg/mL)
4. Maleic acid (0.01 mg/mL)
Conditions
Column : Shim-pack GIS CN (250 mmL. × 4.6 mmI.D., 5 μm)
(P/N: 227-30263-07)
Mobile Phase : 20 mmol/L Monopotassium phosphate buffer solution (pH 4.0)
Flow Rate : 1.0 mL/min
Col. Temp. : 40 ºC
Detection : UV 210 nm
Injection Vol. : 5 μL

Pg A-64