Page 66 - Shimadu Consumables Catalog

UHPLC/HPLC Columns

Shim-pack GIST Amide

Strong retention of polar compounds
This is the amide type HILIC column in the Shim-pack Series. Bonded Phase Amide (Carbamoyl) Groups
It shows stronger retention of polar compounds than other Particle Size 3μm, 5μm
HILIC columns such as unmodified silica and Diol type and Pore Size 10 nm
it provides a different selectivity. It is also highly durable, Surface Area 350 m /g
2
which is a characteristic of the Shim-pack GIST Series. Carbon Loading 15 %
In addition to analysis of oligosaccharides and glycans, End-capping No
Amide type HILIC columns are often used as general HILIC pH Range 2 to 8.5
columns as they are applicable to a wide range of samples. USP Code L68

Analysis Examples

Conditions
Analysis of Sugar Alcohol Column : Shim-pack GIST Amide (5 μm, 250 x 4.6 mm I.D.)
(P/N: 227-30827-07)
Mobile Phase : Acetonitrile/Water = 80/20, v/v
Flow Rate : 1 mL/min
Col. Temp. : 40 ºC
Detection : RI
Injection Vol. : 10 μL

Conditions
Analysis of Oligosaccharides derivatized with 2-AB Column : Shim-pack GIST Amide (5 μm, 250 x 4.6 mm I.D.)
(P/N: 227-30827-07)
Mobile Phase : Acetonitrile
B) 50 mM HCOONH4 (pH 4.4, HCOOH)
A/B = 80/20 – 20 min – 65/35 – 60 min – 50/50 – 10 min – 50/50 (v/v)
Flow Rate : 1 mL/min
Col. Temp. : 50 ºC
Detection : RI
Injection Vol. : 25 μL
Sample : 2-AB Labeled Glucose Homopolymer Ladder

Pg A-62