Page 60 - Shimadu Consumables Catalog

UHPLC/HPLC Columns

Shim-pack GIST PFPP

Strong retention for highly polar basic compounds
Different from other Shim-pack Series PFPP Bonded Phase Pentafluorophenyl propyl Group
(pentafluorophenylpropyl) columns, this PFPP column is Particle Size 3μm, 5μm
end-capped so it provides a different selectivity. Durability is Pore Size 10 nm
a challenge for PFPP columns. However, Shim-pack GIST PFPP Surface Area 350 m /g
2
provides both high durability, which is a characteristic of the Carbon Loading 10 %
Shim-pack GIST Series, and separation characteristics of PFPP End-capping No
via a unique, advanced end-capping technology. pH Range 2 to 7.5

Generally, PFPP columns retain more highly polar basic USP Code L43
compounds. This is more prominent in Shim-pack GIST
PFPP and it also provides an extremely high retention
performance when compared to other commercially-
available PFPP columns.

Analysis Examples
Peaks (100 µg/L each)
Analysis of Antihistamines 1. Ketotifen 5. Triprolidine 9. Loratidine
2. Epinastine 6. Hydroxyzine 10. Homoclorcyclizine
3. Chlorpheniramine 7. Fexofenadine 11. Clemastine
4. Cetirizine 8. Azelastine
Conditions
Column : Shim-pack GIST-HP PFPP (3 μm, 150 x 2.1 mm I.D.)
(P/N: 227-30890-05)
Mobile Phase : A) Acetonitrile
B) 0.1% Formic acid in water
A/B = 40/60, v/v
Flow Rate : 0.2 mL/min
Col. Temp. : 40 ºC
Detection : LCMS-8030 Plus (ESI, Positive, SRM)
Injection Vol. : 1 μL
Sample : Standard

Analytical Columns

Particle Size I.D. (mm) 1.0 1.5 2.1 3.0 4.0 4.6
(μm) Length (mm)
20 - - 227-30858-01 227-30859-01 227-30860-01 227-30861-01
30 227-30856-01 227-30857-01 227-30858-02 227-30859-02 227-30860-02 227-30861-02
50 227-30856-02 227-30857-02 227-30858-03 227-30859-03 227-30860-03 227-30861-03
75 227-30856-03 227-30857-03 227-30858-04 227-30859-04 227-30860-04 227-30861-04
3
100 227-30856-04 227-30857-04 227-30858-05 227-30859-05 227-30860-05 227-30861-05
125 - - 227-30858-06 227-30859-06 227-30860-06 227-30861-06
150 227-30856-05 227-30857-05 227-30858-07 227-30859-07 227-30860-07 227-30861-07
250 227-30856-06 227-30857-06 227-30858-08 227-30859-08 227-30860-08 227-30861-08
20 - - 227-30864-01 227-30865-01 227-30866-01 227-30867-01
30 227-30862-01 227-30863-01 227-30864-02 227-30865-02 227-30866-02 227-30867-02
50 227-30862-02 227-30863-02 227-30864-03 227-30865-03 227-30866-03 227-30867-03
75 227-30862-03 227-30863-03 227-30864-04 227-30865-04 227-30866-04 227-30867-04
5
100 227-30862-04 227-30863-04 227-30864-05 227-30865-05 227-30866-05 227-30867-05
125 - - 227-30864-06 227-30865-06 227-30866-06 227-30867-06
150 227-30862-05 227-30863-05 227-30864-07 227-30865-07 227-30866-07 227-30867-07
250 227-30862-06 227-30863-06 227-30864-08 227-30865-08 227-30866-08 227-30867-08

Pg A-56