Page 58 - Shimadu Consumables Catalog

UHPLC/HPLC Columns

Shim-pack GIST Phenyl-Hexyl

Alternative Selectivity to C18 Columns
Shim-pack GIST Phenyl-Hexyl columns are bonded with a Bonded Phase Phenylhexyl Groups
phenyl ring together with a hexyl (C6) chain, which provides Particle Size 3 µm, 5 µm
complementary selectivity to straight alkyl-chain columns Pore Size 10 nm
due to its π-π interactions and hydrophobic interactions. Surface Area 350 m /g
2
Furthermore, Shim-pack GIST Phenyl-Hexyl maintains the Carbon Loading 9 %
same ultra-high inertness, wide pH range and high durability End-capping Yes
as the Shim-pack GIST C18, achieving stronger retention pH Range 1 to 10
than other phenyl columns as well as reducing or eliminating USP Code L11
adsorption of polar compounds.

Analysis Examples

Conditions
Column : 150 mmL. × 4.6 mmI.D., 5 µm
Mobile Phase : A) Acetonitrile
B) 25 mmol/L Monopotassium phosphate buffer
solution (pH 3.0)
Flow Rate : A/B=45/55 (v/v)
Col. Temp. : 40 ºC
Detection : UV 230 nm
Conditions
Column : 150 mmL. × 4.6 mmI.D., 5 µm
Mobile Phase : A) Water
B) Methanol
A/B = 30/70 (v/v)
Flow Rate : 1.0 mL/min
Col. Temp. : 40 ºC
Detection : UV 254 nm

Pg A-54