Page 54 - Shimadu Consumables Catalog

UHPLC/HPLC Columns

Shim-pack GIST C8

Ultra-High Inertness, High Durability
Shim-pack GIST C8 is packed with high-purity porous Bonded Phase Octyl Group
spherical silica for delivering the same extreme inertness to Particle Size 2 μm, 3 μm, 5 μm
elute either basic or acidic compounds without undesired Pore Size 10 nm
adsorption. Low retentivity and no sample adsorption Surface Area 350 m /g
2
enable analysis of natural samples. Carbon Loading 8 %
Shim-pack GIST C8 is the ideal choice for the rapid analysis of End-capping Yes
hydrophobic compounds. pH Range 1 to 10
USP Code L7
Analysis Examples

Conditions Conditions
Column : Shim-pack GIST C8 (150 mmL. × 4.6 mmI.D., 5 μm) Column : Shim-pack GIST C8 (150 mmL. × 2.1 mmI.D., 3 μm)
(P/N: 227-30137-07) (P/N: 227-30164-07)
Mobile Phase : A) 10 mmol/L Disodium phosphate buffer solution Mobile Phase : 0.1 % Formic acid, 5 mmol/L Ammonium formate in
(pH 8.0) Methanol
B) Tetrahydrofuran Flow Rate : 0.2 mL/min
A/B = 87/13, v/v Col. Temp. : 40 ºC
Flow Rate : 1.0 mL/min Detection : LC/MS/MS (ESI, Negative, MRM)
Col. Temp. : 40 ºC Injection Vol. : 2 μL
Detection : UV 260 nm
Injection Vol. : 5 μL

Analytical Columns

Particle Size I.D. (mm) 1.0 1.5 2.1 3.0 4.0 4.6
(μm) Length (mm)
20 - - 227-30164-01 227-30165-01 227-30166-01 227-30167-01
30 227-30162-01 227-30163-01 227-30164-02 227-30165-02 227-30166-02 227-30167-02
50 227-30162-02 227-30163-02 227-30164-03 227-30165-03 227-30166-03 227-30167-03
75 227-30162-03 227-30163-03 227-30164-04 227-30165-04 227-30166-04 227-30167-04
3
100 227-30162-04 227-30163-04 227-30164-05 227-30165-05 227-30166-05 227-30167-05
125 - - 227-30164-06 227-30165-06 227-30166-06 227-30167-06
150 227-30162-05 227-30163-05 227-30164-07 227-30165-07 227-30166-07 227-30167-07
250 227-30162-06 227-30163-06 227-30164-08 227-30165-08 227-30166-08 227-30167-08
20 - - 227-30170-01 227-30171-01 227-30172-01 227-30173-01
30 227-30168-01 227-30169-01 227-30170-02 227-30171-02 227-30172-03 227-30173-02
50 227-30168-02 227-30169-02 227-30170-03 227-30171-03 227-30172-04 227-30173-03
75 227-30168-03 227-30169-03 227-30170-04 227-30171-04 227-30172-05 227-30173-04
5
100 227-30168-04 227-30169-04 227-30170-05 227-30171-05 227-30172-06 227-30173-05
125 - - 227-30170-06 227-30171-06 227-30172-07 227-30173-06
150 227-30168-05 227-30169-05 227-30170-07 227-30171-07 227-30172-08 227-30173-07
250 227-30168-06 227-30169-06 227-30170-08 227-30171-08 227-30172-09 227-30173-09

Pg A-50