Page 50 - Shimadu Consumables Catalog

UHPLC/HPLC Columns

Shim-pack GIS C18-P

High Steric Selectivity
Shim-pack GIS C18-P is designed with a polymerically Bonded Phase Octadecyl Groups
bonded octadecy group, which provides high steric Particle Size 3 µm, 5 µm
selectivity for separation of planar and non-planar Pore Size 10 nm
compounds. It achieves complete baseline separation of Surface Area 450 m /g
2
structurally similar compounds such as vitamin D2 and Carbon Loading 29 %
D3 because of the planarity recognition capability. End-capping -
Shim-pack GIS C18-P is also ideal for the HPLC analysis pH Range 2 - 7.5
of 16 PAH compounds listed as target pollutants by the USP Code L1
U.S. EPA.

Analysis Examples
Due to increased retention of planar structural compounds, Shim-pack GIS C18-P shows different selectivity compared to Shim-pack
GIS C18.
Peaks
1. Phenanthro[3,4-c]phenanthrene (PhPh)

2. Tetrabenzonaphthalene (TBN)

3. Benzo[a]pyrene (BaP)

Conditions
Column : 250 mmL. × 4.6 mmI.D., 5 µm
Mobile Phase : A) Water
B) Acetonitrile
A/B = 15/85 (v/v)
Flow Rate : 2.0 mL/min
Col. Temp. : 30 ºC
Detection : UV 254 nm
Samples : Standard Reference Material 869

Peaks (100 μg/L each)
1. Vitamin D2 (Calciferol)
2. Vitamin D3 (Cholecarciferol)
Conditions
Column : Shim-pack GIS C18-P (250 mmL. × 4.6 mmI.D., 5 µm)
(P/N: 227-30557-07)
Mobile Phase : Acetonitrile
Flow Rate : 1.5 mL/min
Col. Temp. : 40 ºC
Detection : UV 265 nm
Injection Vol. : 200 µL

Pg A-46