Page 48 - Shimadu Consumables Catalog

UHPLC/HPLC Columns

Shim-pack GIS C18

High Retentivity, Lower Column Back Pressure
Widely used octadecyl bonded silica gel enables the Shim- Bonded Phase Octadecyl Groups
pack GIS C18 to have strong hydrophobic interaction and Particle Size 2µm, 3µm, 4µm, 5µm, 10µm
low absorption of ionic compounds. In addition, highly Pore Size 10 nm
uniform particles ensure stable mobile phase delivery and Surface Area 450 m /g
2
outstanding low pressure. Carbon Loading 15%
Shim-pack GIS C18 is ideal for preparative separations. End-capping Yes
Higher surface area silica and strong retentivity provide high pH Range 2 to 7.5
preparative loading capacity without sacrificing peak shape. USP Code L1
Analysis Examples

Peaks
1. Tartrazine 7.6 mg/L 10. Ponceau SX 5.3 mg/L
2. Amaranth 3.8 mg/L 11. Orange I 5.3 mg/L
3. Ingigocarmine 7.6 mg/L 12. Fast green FCF 3.0 mg/L
4. New coccine 3.8 mg/L 13. Brilliant blue FCF 3.0 mg/L
5. Sunset Yellow FCF 5.3 mg/L 14. Ponceau 3R 7.6 mg/L
6. Naphthol Yellow S 7.6 mg/L 15. Erythrosine 5.3 mg/L
7. Uranine 3.8 mg/L 16. Azure Blue VX 3.0 mg/L
8. Allura Red AC 5.3 mg/L 17. Orange II 7.6 mg/L
9. Ponceau R 7.6 mg/L 18. Acid red 3.0 mg/L
Conditions
Column : Shim-pack GIS C18 (150 mmL. × 4.6 mmI.D, 4 μm)
(P/N: 227-30100-07)
Mobile Phase : A) 10 mmol/L Disodium phosphate buffer solution (pH 6.9)
B) Acetonitrile
A/B = 90/10 - 50 min - 65/35
Flow Rate : 1.0 mL/min
Col. Temp. : 40 ºC
Detection : UV 270 nm
Injection Vol. : 10 μL

Analytical Columns

Particle Size I.D. (mm)
(μm) Length (mm) 1.0 1.5 2.1 3.0 4.0 4.6
33 227-30095-01 227-30096-01 227-30096-05 227-30096-12 227-30096-19 227-30096-26
50 227-30095-02 227-30096-02 227-30096-06 227-30096-13 227-30096-20 227-30096-27
75 227-30095-03 227-30096-03 227-30096-07 227-30096-14 227-30096-21 227-30096-28
3 100 227-30095-04 227-30096-04 227-30096-08 227-30096-15 227-30096-22 227-30096-29
125 - - 227-30096-09 227-30096-16 227-30096-23 227-30096-30
150 - - 227-30096-10 227-30096-17 227-30096-24 227-30096-31
250 - - 227-30096-11 227-30096-18 227-30096-25 227-30096-32
30 - - 227-30097-01 227-30098-01 227-30099-01 227-30100-01
33 - - 227-30097-02 227-30098-02 227-30099-02 227-30100-02
50 - - 227-30097-03 227-30098-03 227-30099-03 227-30100-03
75 - - 227-30097-04 227-30098-04 227-30099-04 227-30100-04
4
100 - - 227-30097-05 227-30098-05 227-30099-05 227-30100-05
125 - - 227-30097-06 227-30098-06 227-30099-06 227-30100-06
150 - - 227-30097-07 227-30098-07 227-30099-07 227-30100-07
250 - - 227-30097-08 227-30098-08 227-30099-08 227-30100-08
30 - - 227-30103-01 227-30104-01 227-30105-01 227-30106-01
33 227-30101-01 227-30102-01 227-30103-02 227-30104-02 227-30105-02 227-30106-02
50 227-30101-02 227-30102-02 227-30103-03 227-30104-03 227-30105-03 227-30106-03
75 227-30101-03 227-30102-03 227-30103-04 227-30104-04 227-30105-04 227-30106-04
5
100 227-30101-04 227-30102-04 227-30103-05 227-30104-05 227-30105-05 227-30106-05
125 - - 227-30103-06 227-30104-06 227-30105-06 227-30106-06
150 227-30101-05 227-30102-05 227-30103-07 227-30104-07 227-30105-07 227-30106-07
250 227-30101-06 227-30102-06 227-30103-08 227-30104-08 227-30105-08 227-30106-08

Pg A-44