Page 44 - Shimadu Consumables Catalog

UHPLC/HPLC Columns

Shim-pack GIST C18-AQ

Excellent Retentivity of Highly Polar Compounds
Shim-pack GIST C18-AQ achieves strong retention of Bonded Phase Octadecyl Groups
hydrophilic highly polar compounds compared to general Particle Size 1.9 µm, 3 µm, 5 µm
C18 columns, while maintaining high inertness and durability Pore Size 10 nm
in highly or 100% aqueous mobile phases. Surface Area 350 m /g
2
Shim-pack GIST C18-AQ is also able to reduce the absorption Carbon Loading 13 %
of basic and acidic compounds and achieve superior peak End-capping Yes
shapes in the analysis of metal complexes. pH Range 1 to 10
USP Code L1, L96

Analysis Examples

Conditions Conditions
Column : Shim-pack GIST C18-AQ (100 mmL.× 2.1 mmI.D., 1.9 µm) Column : Shim-pack GIST C18-AQ (150 mmL. × 4.6 mmI.D., 5 µm)
(P/N: 227-30807-02) (P/N: 227-30742-07)
Mobile Phase : A) 0.1 % Formic acid in Water Mobile Phase : A) 0.1 % Formic acid in Water
B) Acetonitrile B) Acetonitrile
A/B = 100/0 - 0.2 min - 100/0 - 0.5 min - 15/85 - 1.5 min - A/B = 80/20 (v/v)
15/85 - 1.52 min - 100/0 - 2.5 min - 100/0 (v/v) Flow Rate : 1.0 mL/min
Flow Rate : 0.8 mL/min Col. Temp. : 40 ºC
Col. Temp. : 40 ºC Detection : UV 280 nm
Detection : LC/MS/MS (LCMS-8030, ESI, Positive, SRM)
Injection Vol. : 2 µL

Analytical Columns
Particle Size I.D. (mm) 1.0 1.5 2.1 3.0 4.0 4.6
(μm) Length (mm)
20 - - 227-30721-01 227-30722-01 227-30723-01 227-30724-01
30 227-30719-01 227-30720-01 227-30721-02 227-30722-02 227-30723-02 227-30724-02
50 227-30719-02 227-30720-02 227-30721-03 227-30722-03 227-30723-03 227-30724-03
75 227-30719-03 227-30720-03 227-30721-04 227-30722-04 227-30723-04 227-30724-04
3
100 227-30719-04 227-30720-04 227-30721-05 227-30722-05 227-30723-05 227-30724-05
125 - - 227-30721-06 227-30722-06 227-30723-06 227-30724-06
150 227-30719-05 227-30720-05 227-30721-07 227-30722-07 227-30723-07 227-30724-07
250 227-30719-06 227-30720-06 227-30721-08 227-30722-08 227-30723-08 227-30724-08
20 - - 227-30739-01 227-30740-01 227-30741-01 227-30742-01
30 227-30737-01 227-30738-01 227-30739-02 227-30740-02 227-30741-02 227-30742-02
50 227-30737-02 227-30738-02 227-30739-03 227-30740-03 227-30741-03 227-30742-03
75 227-30737-03 227-30738-03 227-30739-04 227-30740-04 227-30741-04 227-30742-04
5
100 227-30737-04 227-30738-04 227-30739-05 227-30740-05 227-30741-05 227-30742-05
125 - - 227-30739-06 227-30740-06 227-30741-06 227-30742-06
150 227-30737-05 227-30738-05 227-30739-07 227-30740-07 227-30741-07 227-30742-07
250 227-30737-06 227-30738-06 227-30739-08 227-30740-08 227-30741-08 227-30742-08

Pg A-40