Page 42 - Shimadu Consumables Catalog

UHPLC/HPLC Columns

Shim-pack GIST C18

Ultra High Inertness, High Durability
Shim-pack GIST C18 has superior inertness, which improves Bonded Phase Octadecyl Groups
analysis precision and increases column durability. In Particle Size 2 μm, 3 μm, 5 μm
addition, it can be used to analyze strong ionic compounds Pore Size 10 nm
and difficult to absorb samples, which helps to obtain Surface Area 350 m /g
2
symmetrical peaks and high reproducibility. Carbon Loading 14 %
Furthermore, because of the new silica gel, Shim-pack GIST End-capping Yes
C18 is suitable for wider pH (1-10) analysis at a consistent pH Range 1 to 10
performance. This enables use of a silica-based column under USP Code L1
alkaline conditions.

Analysis Examples

Conditions Conditions
Column : Shim-pack GIST-HP C18 (150 mmL. × 3.0 mmI.D., 3 μm) Column : Shim-pack GIST C18 (150 mmL. × 4.6 mmI.D., 5 μm)
(P/N: 227-30040-05) (P/N: 227-30017-07)
Mobile Phase : A) 50 mM KH2PO4 (pH 3.5, H3PO4) Mobile Phase : 0.1 mol/L Ammonium phosphate, 0.2 mol/L Sodium
B) Acetonitrile perchlorate buffer solution (pH 2.0)
A/B = 60/40 - 4 min - 40/60 Flow Rate : 1.0 mL/min
Flow Rate : 1.5 mL/min Col. Temp. : 40 ºC
Col. Temp. : 40 ºC Detection : UV 260 nm
Detection : UV 235 nm Injection Vol. : 1 μL
Injection Vol. : 5.0 μL

Analytical Columns

Particle Size I.D. (mm) 1.0 1.5 2.1 3.0 4.0 4.6
(μm) Length (mm)
20 - - 227-30008-01 227-30009-01 227-30010-01 227-30011-01
30 227-30006-01 227-30007-01 227-30008-02 227-30009-02 227-30010-02 227-30011-02
50 227-30006-02 227-30007-02 227-30008-03 227-30009-03 227-30010-03 227-30011-03
75 227-30006-03 227-30007-03 227-30008-04 227-30009-04 227-30010-04 227-30011-04
3
100 227-30006-04 227-30007-04 227-30008-05 227-30009-05 227-30010-05 227-30011-05
125 - - 227-30008-06 227-30009-06 227-30010-06 227-30011-06
150 227-30006-05 227-30007-05 227-30008-07 227-30009-07 227-30010-07 227-30011-07
250 227-30006-06 227-30007-06 227-30008-08 227-30009-08 227-30010-08 227-30011-08
20 - - 227-30014-01 227-30015-01 227-30016-01 227-30017-01
30 227-30012-01 227-30013-01 227-30014-02 227-30015-02 227-30016-02 227-30017-02
50 227-30012-02 227-30013-02 227-30014-03 227-30015-03 227-30016-03 227-30017-03
75 227-30012-03 227-30013-03 227-30014-04 227-30015-04 227-30016-04 227-30017-04
5
100 227-30012-04 227-30013-04 227-30014-05 227-30015-05 227-30016-05 227-30017-05
125 - - 227-30014-06 227-30015-06 227-30016-06 227-30017-06
150 227-30012-05 227-30013-05 227-30014-07 227-30015-07 227-30016-07 227-30017-07
250 227-30012-06 227-30013-06 227-30014-08 227-30015-08 227-30016-08 227-30017-08

Pg A-38