Page 46 - Shimadu Consumables Catalog

UHPLC/HPLC Columns

Shim-pack GISS C18

Ideal for Rapid Analysis
Shim-pack GISS C18 maintains the same ultra-high Bonded Phase Octadecyl Groups
inertness and wider pH range as the Shim-pack GIST Particle Size 1.9 μm, 3 μm, 5 μm
C18, while providing rapid separations with symmetrical Pore Size 20 nm
peaks. Surface Area 200 m /g
2
Carbon Loading 9 %
The optimization of surface area, pore size and chemical
bonding delivers superior peak shapes. It is ideal for LC/ End-capping Yes
MS/MS analysis and enables MS-compatible buffers to pH Range 1 to 10
be used due to extremely inert silica gel. USP Code L1

Analysis Examples
Peaks (100 μg/L each)
Q1 > Q3
1. Acebutolol : 337.10 > 116.05 (+)
2. Atenolol : 267.25 > 145.00 (+)
3. Labetalol : 329.00 > 161.95 (+)
4. Nadolol : 310.05 > 254.00 (+)
5. Pindolol : 249.80 > 116.00 (+)
Conditions
Column : Shim-pack GISS C18 (50 mmL × 2.1 mmI.D., 1.9 μm)
(P/N: 227-30048-01)
Mobile Phase : A) 10 mM Ammonium Formate in Water
B) 10 mM Ammonium Formate in Methanol
A/B = 70/30 - 0.3 min - 40/60 - 0.5 min - 0/100 - 0.1 min -
0/100 - 0.01 min - 70/30 - 0.5 min - 70/30, v/v
Flow Rate : 0.6 mL/min
Col. Temp. : 40 °C
Detection : LC/MS/MS (ESI, Positive, Negative MRM)

Analytical Columns

Particle Size I.D. (mm) 1.0 1.5 2.1 3.0 4.0 4.6
(μm) Length (mm)
30 227-30050-01 227-30051-01 227-30052-01 227-30053-01 227-30054-01 227-30055-01
50 227-30050-02 227-30051-02 227-30052-02 227-30053-02 227-30054-02 227-30055-02
75 227-30050-03 227-30051-03 227-30052-03 227-30053-03 227-30054-03 227-30055-03
3 100 227-30050-04 227-30051-04 227-30052-04 227-30053-04 227-30054-04 227-30055-04
125 - - 227-30052-05 227-30053-05 227-30054-05 227-30055-05
150 227-30050-05 227-30051-05 227-30052-06 227-30053-06 227-30054-06 227-30055-06
250 227-30050-06 227-30051-06 227-30052-07 227-30053-07 227-30054-07 227-30055-07
30 227-30056-01 227-30057-01 227-30058-01 227-30059-01 227-30060-01 227-30061-01
50 227-30056-02 227-30057-02 227-30058-02 227-30059-02 227-30060-02 227-30061-02
75 227-30056-03 227-30057-03 227-30058-03 227-30059-03 227-30060-03 227-30061-03
5 100 227-30056-04 227-30057-04 227-30058-04 227-30059-04 227-30060-04 227-30061-04
125 - - 227-30058-05 227-30059-05 227-30060-05 227-30061-05
150 227-30056-05 227-30057-05 227-30058-06 227-30059-06 227-30060-06 227-30061-06
250 227-30056-06 227-30057-06 227-30058-07 227-30059-07 227-30060-07 227-30061-07

Pg A-42