Page 39 - Shimadu Consumables Catalog

Example of the Batch Analysis of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs)
Conditions
Column : Top : Shim-pack VP-Phenyl (150 mmL. × 4.6 mmI.D., 5 μm)
(P/N: 228-59928-91)
Bottom : Shim-pack XR-Phenyl (50 mmL. × 3.0 mmI.D., 2.2 μm)
(P/N: 228-59904-92)
Mobile Phase : A) 20 mmol/L Phosphoric acid (Na) buffer (pH 2.5)
B) Acetonitrile
A/B = 3/7 (v/v)
Flow Rate : Top : 1.0 mL/min
Bottom : 1.0 mL/min
Relative Retention Times
Peak Shim-pack VP-Phenyl Shim-pack XR-Phenyl
1. Piroxicam 1.000 1.000
2. Ketoprofen 1.480 1.483
3. Diflunisal 2.233 2.172
4. Diclofenac 2.949 2.916
5. Indomethacin 3.263 3.262
6. Ibuprofen 3.470 3.501

Columns with the same size but different bonded phases are available as a set for the purpose of method development.
Please contact your local Shimadzu representative for details.

Check out the Nexera Method Scouting System at
www.shimadzu.com/an/hplc

Pg A-35