Page 38 - Shimadu Consumables Catalog

UHPLC/HPLC Columns

Shim-pack VP Series

Smooth Transfer of Methods from High-Speed Analysis to Conventional Analysis
By using the Shim-pack VP series of conventional columns in combination with the Shim-pack XR series of high-speed columns,
which offer the equivalent separation performance, it is possible to perform method development via high-speed analysis, and
then smoothly transfer to conventional analysis. Utilizing these columns with Nexera Method Scouting provides strong support
for method development.

Example of High-Speed/Conventional Analysis Using Columns with Equivalent Retention Behavior

Examples of analysis with the Shim-pack VP and Shim-pack XR columns are shown below. In each example, analysis was achieved
while maintaining essentially the same relative retention time. In method transfer, this equivalency is important, making the
combination of the Shim-pack VP series and Shim-pack XR series optimal for method development.

Example of the Batch Analysis of 6 Sulfa Drugs
Conditions
Column : Top : Shim-pack VP-ODS (150 mmL. × 4.6 mmI.D., 5 μm)
(P/N: 228-34937-91)
Bottom : Shim-pack XR-ODS (50 mmL. × 3.0 mmI.D., 2.2 μm)
(P/N: 228-41606-92)
Mobile Phase : A) 0.1 % formic acid
B) Acetonitrile
A/B = 8/2 (v/v)
Flow Rate : Top : 1.0 mL/min
Bottom : 1.0 mL/min
Relative Retention Times
Peak Shim-pack VP-ODS Shim-pack XR-ODS
1. Sulfadiamine 1.000 1.000
2. Sulfamerazine 1.252 1.238
3. Sulfadimidine 1.537 1.509
4. Sulfamethoxazole 2.809 2.699
5. Sulfaquinoxaline 5.668 5.432

Example of the Analysis of Fat-Soluble Vitamins
Conditions
Column : Top : Shim-pack VP-C8 (150 mmL. × 4.6 mmI.D., 5 μm)
(P/N: 228-59927-91)
Bottom : Shim-pack XR-C8 (50 mmL. × 3.0 mmI.D., 2.2 μm)
(P/N: 228-59902-92)
Mobile Phase : Methanol
Flow Rate : Top : 1.0 mL/min
Bottom : 1.0 mL/min
Relative Retention Times
Peak Shim-pack VP-C8 Shim-pack XR-C8
1. Retinol 1.000 1.000
2. α±-tocopherol 1.509 1.531

Pg A-34