Page 33 - Shimadu Consumables Catalog

Analysis Examples

Analysis of Fat-Soluble Vitamins
The fat-soluble vitamins E (tocopherol) and A (retinol) were analyzed. The Shim-pack XR-C8 column achieves higher speed than a
conventional C8 column.

Conditions
Column : Shim-pack XR-C8 (75 mmL. × 3.0 mmI.D., 2.2 µm)
(P/N: 228-59902-93)
Mobile Phase : Methanol
Flow Rate : 1.0 mL/min
Col. Temp. : 40 °C
Detection : UV 290 nm

Analysis of Non-Steroidal Anti-Inflammatory Drug
The column was switched from Shim-pack XR-ODS to Shim-pack XR-Phenyl to improve the resolution.
The difference in retention properties between the ODS group and the phenylpropyl group improves the peak shape, controls the
resolution, and reduces the analysis time.

Conditions
Column : Shim-pack XR-Phenyl (75 mmL. × 3.0 mmI.D., 2.2 µm)
(P/N: 228-59904-93)
Mobile Phase : A) 20 mmol/L Phosphate buffer solution (pH 2.5)
B) Acetonitrile
A/B = 30/20 (v/v)
Flow Rate : 1.0 mL/min
Col. Temp. : 40 °C
Detection : UV 220 nm

Fast Normal-Phase Analysis
Organic solvents are used as the mobile phase for normal-phase analysis. Due to environmental considerations, it is necessary to
reduce the consumption of mobile phase compared with normal reversed-phase analysis. The Shim-pack XR-SIL silica column increases
the speed of normal-phase analysis while reducing the consumption of mobile phase. In this example, the analysis time is reduced by
80 % while maintaining the flow rate, thereby reducing the overall mobile phase consumption to 20 % or less.

Conditions
Column : Shim-pack XR-SIL (75 mmL. × 3.0 mmI.D., 2.2 µm)
(P/N: 228-59906-92)
Mobile Phase : Hexane / Ethanol = 90/10 (v/v)
Flow Rate : 1.0 mL/min
Col. Temp. : 40 °C
Detection : UV 254 nm

Pg A-29