Page 31 - Shimadu Consumables Catalog

High Peptide Recovery enabled by Shim-pack Arata technology

Since the adsorption of peptides to particles is minimized, high recovery of peptides is ensured and excellent peptide analysis can
be provided by Shim-pack Arata Peptide C18 column.

Analysis of Tryptic Digest of Myoglobin

Peptides are known to show non-specific adsorption to particles of LC columns. A Shim-pack Arata Peptide C18 column showed
high recovery of peptides.

Analytical Conditions
LC System : NexeraX2MP_M30A (STD Cell) Detection : 214 nm
LC Column : Shim-pack Arata Peptide C18 (2.0 × 150 mm, 2.2 μm) Column temp. : 40 °C
Core shell Peptide C18 (2.1×150 mm, sub 2 μm) Injection volume : 1 μL
Core shell C8 (2.1 ×150 mm, sub 4 μm) Sample : Angiotensin I
Mobile Phase A : 0.1% HCOOH in H2O Vial : Torast-H Bio Vial
Mobile Phase B : 0.1% HCOOH in CH3CN
Gradient : 2%B (0-5 min)45%B (20 min)100%B (20.01 – 25 min)
2%B (25.01 – 30 min)

Tryptic digest of myoglobin was analyzed using Shim-pack Arata Peptide C18 (2.2 μm), Core shell C18 (sub 2 μm) and a
Core shell C8 (sub 4 μm). Compared to the other two columns, Shim-pack Arata Peptide C18 showed significantly larger
peak area, which suggests that higher recovery could be obtained with Shim-pack Arata Peptide C18 column.

Shim-pack Arata Peptide C18

Particle Size I.D. (mm) 2.0
(μm) Length (mm)
50 227-32806-01
2.2 100 227-32806-02
150 227-32806-03

Pg A-27