Page 52 - Shimadu Consumables Catalog

UHPLC/HPLC Columns

Shim-pack GIS RP-Shield

Embedded with a Polar Functional Group
Shim-pack GIS RP-Shield contains a polar functional Bonded Phase Octadecyl Groups
group embedded between silica surface and an Particle Size 5 μm
octadecyl group, making it stable in 100% aqueous Pore Size 10 nm
mobile phases without phase collapse. The embedded Surface Area 450 m /g
2
polar functional group is also extremely base Carbon Loading 9 %
deactivated, which enables the column to provide End-capping -
superior peak shape for acids. pH Range 2 - 7.5

Shim-pack GIS RP-Shield provides unique selectivity as USP Code L1
hydrogen bonding interactions, making it suitable for
separations that cannot be achieved by other modes,
such as hydrophobic interactions or π-π interactions.

Analysis Examples
Peaks
1. Phenoxyacetic acid
2. Benzoic acid

Conditions
Column : Shim-pack GIS RP-Shield (150 mmL. × 3.0 mmI.D., 5 µm)
(P/N: 227-30587-06)
Mobile Phase : A) 0.1 % Formic acid in Water
B) Acetonitrile
A/B = 50/50 (v/v)
Flow Rate : 0.4 mL/min
Col. Temp. : 40 °C
Detection : UV 254 nm

Analytical Columns

Particle Size I.D. (mm) 1.0 1.5 2.1 3.0 4.0 4.6
(μm) Length (mm)
30 - - 227-30586-01 227-30587-01 227-30588-01 227-30589-01
33 227-30584-01 227-30585-01 227-30586-02 227-30587-02 227-30588-02 227-30589-02
50 227-30584-02 227-30585-02 227-30586-03 227-30587-03 227-30588-03 227-30589-03
5 75 227-30584-03 227-30585-03 227-30586-04 227-30587-04 227-30588-04 227-30589-04
100 227-30584-04 227-30585-04 227-30586-05 227-30587-05 227-30588-05 227-30589-05
150 227-30584-05 227-30585-05 227-30586-06 227-30587-06 227-30588-06 227-30589-06
250 227-30584-06 227-30585-06 227-30586-07 227-30587-07 227-30588-07 227-30589-07

Cartridge Guard Columns
Particle Size I.D. (mm) Cartridge Guard Column (2pcs) Holder
(μm) Length (mm) 1.0 1.5 3.0 4.0
10 227-30612-01 227-30613-01 227-30614-01 227-30616-01 227-30532-01
5
20 - - 227-30615-01 227-30617-01 227-30532-02
Particle Size I.D. (mm) Cartridge Guard Column (2pcs) and Holder
(μm) Length (mm) 1.0 1.5 3.0 4.0
10 227-30612-02 227-30613-02 227-30614-02 227-30616-02
5
20 - - 227-30615-02 227-30617-02

Pg A-48