Page 56 - Shimadu Consumables Catalog

UHPLC/HPLC Columns

Shim-pack GIST Phenyl

Extremely Strong π-π Interactions
The extremely unique phase characteristics of Shim-pack Bonded Phase Phenyl Group
GIST Phenyl are critical to resolving compounds that could Particle Size 2 μm, 3 μm, 5 μm
not be separated with a C18 or C8 phase column. Pore Size 10 nm
Surface Area 350 m /g
2
In addition to π-π interactions, Shim-pack GIS Phenyl
Carbon Loading 10 %
provides hydrogen bonding secondary interactions, which
results in retaining polar compounds at the same time. As End-capping -
the phenyl groups are directly bonded to the silica gel, Shim- pH Range 2 to 7.5
pack GIS Phenyl is also capable of the analysis of structural USP Code L11
isomers due to its high stereo-selectivity.

Analysis Examples

Peaks (0.1 mg/L each)
1. Hydrocortisone
2. Prednisolone
Conditions
Column : Shim-pack GIST Phenyl (50 mmL. × 2.1 mmI.D., 2 μm)
(P/N: 227-30207-02)
Mobile Phase : A) 0.05 % Formic acid in Water
B) 0.05 % Formic acid in Methanol
A/B = 60/40, v/v
Flow Rate : 0.6 mL/min
Col. Temp. : 40 ºC
Detection : LC/MS/MS (ESI, Positive, MRM)
Injection Vol. : 5 μL

Analytical Columns

Particle Size I.D. (mm)
(μm) Length (mm) 1.0 1.5 2.1 3.0 4.0 4.6
20 - - 227-30211-01 227-30212-01 227-30213-01 227-30214-01
30 227-30209-01 227-30210-01 227-30211-02 227-30212-02 227-30213-02 227-30214-02
50 227-30209-02 227-30210-02 227-30211-03 227-30212-03 227-30213-03 227-30214-03
3 75 227-30209-03 227-30210-03 227-30211-04 227-30212-04 227-30213-04 227-30214-04
100 227-30209-04 227-30210-04 227-30211-05 227-30212-05 227-30213-05 227-30214-05
150 227-30209-05 227-30210-05 227-30211-06 227-30212-06 227-30213-06 227-30214-06
250 227-30209-06 227-30210-06 227-30211-07 227-30212-07 227-30213-07 227-30214-07
20 - - 227-30217-01 227-30218-01 227-30219-01 227-30220-01
30 227-30215-01 227-30216-01 227-30217-02 227-30218-02 227-30219-02 227-30220-02
50 227-30215-02 227-30216-02 227-30217-03 227-30218-03 227-30219-03 227-30220-03
5 75 227-30215-03 227-30216-03 227-30217-04 227-30218-04 227-30219-04 227-30220-04
100 227-30215-04 227-30216-04 227-30217-05 227-30218-05 227-30219-05 227-30220-05
150 227-30215-05 227-30216-05 227-30217-06 227-30218-06 227-30219-06 227-30220-06
250 227-30215-06 227-30216-06 227-30217-07 227-30218-07 227-30219-07 227-30220-08

Pg A-52