Page 85 - Application Notebook - Solution for Food Safety
P. 85
Application No.C141
News
Q Peanut Allergen in Other Nuts
Walnuts, cashew nuts, and almonds were analyzed to test specificity. These nuts were spiked with 2 ppm (2 mg/kg) of
peanut before sample preparation. The spiked peanut peptides were successfully detected and any obvious peak was
detected in blank samples.
Walnut Cashew Almond
(×1,000) (×10,000) (×1,000)
1.0
5.0 7.5
Peanut spiked 0.5 5.0
(2 ppm) 2.5
2.5
0.0 0.0 0.0
4.00 4.25 4.00 4.25 4.00 4.25
(×1,000) (×10,000) (×1,000)
1.0
5.0 7.5
Blank 0.5 5.0
sample 2.5
2.5
0.0 0.0 0.0
4.00 4.25 4.00 4.25 4.00 4.25
Fig. 6 Chromatograms of Peptide GTG… in Other Kind of Nuts With or Without Spiking with Peanuts
Q Detection of ARA h1 in Spice Mixes and Seasonings
Several spice mixes and seasonings were analyzed using sample preparation and analytical conditions described here.
Peaks of tryptic peptides of Ara h1 from samples without spiking of peanut peptides were detected.
(×1,000) (×1,000)
3.0
5.0
2.0
2.5
1.0
0.0 0.0
4.00 4.25 4.00 4.25
Chili mix Taco seasoning
Fig. 7 Detected Peaks of Peptide GTG… in Chili Mix and Seasoning
