Page 89 - Application Notebook - Solution for Food Safety

Application No. AD-0095
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Analysis of Beverage Samples  Conclusions
The UHPLC method established was applied for A rapid and high sensitivity UHPLC method for
quantitation of preservatives in three kinds of beverages: quantitation of six preservatives, benzoic acid, sorbic acid
soft drink B1, fruit juice B2 and cocoa drink B3. The and four para-hydroxybenzoic acid esters (parabens), in
chromatograms of the samples are shown in Fig. 4 and beverages was established using a reversed phase UHPLC
the results are summarized into Table 5. No preservatives column (1.9 μm particle size). A capillary flow cell with
was detected in cocoa drink. Benzoic acid and sorbic acid extra long optical path of 85 mm was employed in the
were detected in the soft drink and fruit juice. The photodiode array detector. The method achieves LOQs
identification of both benzoic acid and sorbic acid peaks ranging 8 to 10 μg/L for the compounds except
were confirmed by UV spectra. benzoic acid (280 μg/L), with 1 μL injection volume.
The very small injection volume minimizes the
contamination of beverage samples to the column and
system, as such suitable for direct analysis of beverage
samples without need for clean up procedure.

<References>
1
* : Singapore Agri-Food and Veterinary Services website: Food
Regulations – AVA (2014)
2
* : United States Department of Agriculture Food Safety and
Inspection Service, Office of Public Health Science: Determination
of Benzoic acid, Sorbic acid and Methyl, Ethyl, Propyl and Butyl
Parabens by HPLC (2004)
3
* : Tzu-Yun Chu, Chine-Lin Chen and Hsueh-Fang Wang, A Rapid
Method for the Simultaneous Determination of Preservatives in Soy
Sauce, Journal of Food and Drug Analysis, Vol. 11, No. 3, Pages
246-250 (2003)
4
* : Shu-Xiang Sun, Shu-Bai Li and Pei Yao, Simultaneous Determination
of Six Preservatives in Beverage by HPLC, 3rd International Conference
on Material, Mechanical and Manufacturing Engineering (2015)

Chromatogram of Beverage Samples (Injection: 1 mL):
Soft Drink with 20 Times Dilution (top);
Fruit Drink with 2 Times Dilutions;
Cocoa Drink without Dilution (Bottom)

Table 5 Quantitative Results of Six Preservatives in Three
Beverages, Each with Duplicate Injections
Benzoicacid Sorbicacid Parabens
Sample
Name RT Conc. RT Conc. RT Conc.
(min) (mg/L) (min) (mg/L) (min) (mg/L)
B1 1.7 82.4 2.18 137.4
B2 1.7 142.4 2.18 13.8 ND
B3 ND

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