Page 90 - Application Notebook - Solution for Food Safety

Application Food Safety Analysis / Nexera X2

News
Development of an UHPLC Method for
Simultaneous Determination of Thirteen
No. AD-0096 Bisphenols in Milk Samples

 Introduction
Bisphenol A (BPA) and Bisphenol F (BPF) are the listed in the EU legislation No 1895/2005 on the restriction
monomers to make polycarbonate plastics and epoxy of use of certain epoxy derivatives in materials contacted
resins. Their diglycidyl esters, i.e., BADGE and BFDGE with food. BPA has estrogenic effect and can disrupt
(Fig. 1), are also present in the polymeric products. These normal hormone levels and development in fetuses
materials are made into a variety of consumer products and babies. In U.S., FDA has published food additive
or used as inner coatings for baby milk bottles and regulations prohibiting the use of BPA-based epoxy
reusable food containers which are allowed to use in resins as inner coatings of containers for infant formula
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refrigerator and microwave for food storage and heating. packaging * . We describe in this Application News a
It has been reported that polycarbonate plastics and new UHPLC method for simultaneous determination of
epoxy-based coatings can release BPA, BPF BADGE and thirteen concerned bisphenols including BPA, BPF,
BFDGE as well as their reaction products as illustrated in BADGE, BFDGE and some structural analogues. An
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Fig. 1 * . These leached chemicals can migrate into food UHPLC system (Nexera X2, Shimadzu Corporation) with
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and become contaminants consumed by consumers a high sensitivity fluorescence detector * was adopted
eventually. Although researches indicate that the to develop a fast and high sensitivity method to meet
migration of these chemicals is normally extremely low * , the requirements of regulations.
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the specific migration limits (SMLs) of bisphenols were

Chemical Structures and Abbreviation Names of Bisphenol A,
Bisphenol F, Their Diglycidyl Esters and Derivatives * 1

*: positional isomers of BFDGE-2 and BFDGE-2HCl-2 are not shown.