Page 82 - Application Notebook - Solution for Food Safety

SCA_210_041

 Calibration Area
3.400e6 y = 67657490x + 6167.372
R² = 0.9989034 R = 0.9994516
3.200e6 Curve Fit: Default (Linear)
The matrix matched calibration curve (Figure 3-6) 3.000e6 Weighting: Default (1/C)
Zero: Default (Not Forced)
was prepared according to the method described 2.800e6 Mean RF: 7.093854e+007
before ranging from 0.0005 mg/kg to 0.05 mg/kg. 2.600e6 SD RF: 3.424479e+006
2.400e6 %RSD: 4.827389
Control samples at 0.001 mg/kg and 0.01 mg/kg 2.200e6
correspond to the calibration curve. 2.000e6
Area 1.800e6
y = 26804740x + 2767.185 1.600e6
1.300e6 R² = 0.9898439 R = 0.9949090
1.400e6
Curve Fit: Default (Linear)
1.200e6 1.200e6
Weighting: Default (1/C)
Zero: Default (Not Forced) 1.000e6
1.100e6
Mean RF: 2.827688e+007 8.000e5
1.000e6 SD RF: 2.951362e+006 Control samples: 0.01 mg/kg
%RSD: 10.437367 6.000e5
9.000e5
4.000e5
8.000e5 2.000e5
Control samples: 0.001 mg/kg
0.000e0
7.000e5 0.000 0.005 0.010 0.015 0.020 0.025 0.030 0.035 0.040 0.045 0.050
Conc. (mg/kg)
6.000e5
5.000e5 Figure 5: Calibration curve of Fipronil-sulfone in egg ranging from
0.0005 mg/kg to 0.05 mg/kg
4.000e5
Area
3.000e5 2.800e6 y = 50916160x + 5542.805
Control samples: 0.01 mg/kg R² = 0.9986046 R = 0.9993021
2.000e5 2.600e6
Curve Fit: Default (Linear)
2.400e6 Weighting: Default (1/C)
1.000e5
Zero: Default (Not Forced)
Control samples: 0.001 mg/kg 2.200e6
0.000e0 Mean RF: 5.386494e+007
0.000 0.005 0.010 0.015 0.020 0.025 0.030 0.035 0.040 0.045 0.050 SD RF: 2.93148e+006
Conc. (mg/kg) 2.000e6 %RSD: 5.442279
Figure 3: Calibration curve of Fipronil in egg ranging from
0.0005 mg/kg to 0.05 mg/kg 1.800e6
Area 1.600e6
y = 63084160x + 1031.500
3.200e6 R² = 0.9992798 R = 0.9996398 1.400e6
3.000e6 Curve Fit: Default (Linear) 1.200e6
Weighting: Default (1/C)
2.800e6 Zero: Default (Not Forced) 1.000e6
2.600e6 Mean RF: 6.363292e+007 8.000e5
SD RF: 2.641896e+006
2.400e6
%RSD: 4.151775 6.000e5
2.200e6 Control samples: 0.01 mg/kg
4.000e5
2.000e6
1.800e6 2.000e5
1.600e6 0.000e0 Control samples: 0.001 mg/kg
0.000 0.005 0.010 0.015 0.020 0.025 0.030 0.035 0.040 0.045 0.050
1.400e6
Conc. (mg/kg)
1.200e6
1.000e6 Figure 6: Calibration curve of Fipronil-desulfinyl in egg ranging from
0.0005 mg/kg to 0.05 mg/kg
8.000e5
6.000e5 Control samples: 0.01 mg/kg
4.000e5  Conclusion
2.000e5
Control samples: 0.001 mg/kg
0.000e0
0.000 0.005 0.010 0.015 0.020 0.025 0.030 0.035 0.040 0.045 0.050 By using the LC/MS/MS method package for
Conc. (mg/kg)
residual pesticides V2 and a QuEChERS sample
Figure 4: Calibration curve of Fipronil-sulfide in egg ranging from preparation a method for the determination of
0.0005 mg/kg to 0.05 mg/kg
Fipronil and Fipronil-sulfone in eggs below the
requested MRL of 0.005 mg/kg could be set up
[1] EU Comission Regulation No 1127/2014 of 20 October 2014 Amending Annexes II and
III to Regulation (EC) No 396/2005 of the European Parliament and of the Council in rapidly without further method development.
regards to maximum residual levels for amitrole, dinocap, fipronil, flufenacet,
pendimethalin, propyzamide and pyridate in or on certain products.
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