Page 81 - Application Notebook - Solution for Food Safety

SCA_210_041

LC system Nexera X2 (Shimadzu, Japan) Mass spectrometer LCMS-8060 (Shimadzu, Japan)
Analytical column Raptor Biphenyl TM Interface voltage -3 kV
100 x 2.1 mm, 2.7 µm (RESTEK) Q1 resolution Unit (0.7 Da FWHM)
Column oven temperature 35 °C
Q3 resolution Unit (0.7 Da FWHM)
Injection volume 2 µl
Nebulizing gas flow 3 L/min
Mobile Phase A 2 mM ammonium formate Drying gas flow 10 L/min
+ 0.002% formic acid - Water
Mobile Phase B 2 mM ammonium formate Heating gas flow 10 L/min
+ 0.002% formic acid - Methanol DL temperature 150 °C
Heat block temperature 300 °C
Interface Temperature 350 °C

In addition, the so-called "MRM spectrum mode"
was used for analysis. Here, not only the
fragments of the quantifier and the qualifiers are
determined, but also a higher number (typically 6-
10) of MRM fragment ions. Using this MRM
spectrum mode, conventional MRM quantification
is combined with a high-quality MRM product ion
spectrum, which can be used in a library search
routine, thus increasing the specificity and
verification of results (Figures 1 and 2).

Figure 2: The figure shows MRM chromatograms for Fipronil, one
recorded with the usual 2 fragment ions, and compared
with a method with higher number (12) of fragment ions
which, despite this fact, have the same sensitivity.

Figure 1: Result of the library search, presented with LabSolutions Insight Screening software