Page 67 - Application Notebook - Solution for Food Safety

Application No.L510
News

Q Similarity Calculation Using UV Spectral Library mAU
All target compounds in this Application News can be 0.6 247 Library
analyzed qualitatively based on UV spectra as well as
0.5
retention times. Sample spectra can be also checked for Matrix Standard
0.4
similarity against library spectra. Fig. 3 shows a UV
spectrum of sulfaquinoxaline in a beef matrix spiked 0.3
with a standard solution of sulfaquinoxaline at 0.2
threshold concentration. Degree of similarity with the
0.1
library spectrum was 0.997.
0.0
250 300 350 400 450 nm
Fig. 3 Spectra of Sulfaquinoxaline

$IJDLFO
uV (×1,000) uV (×1,000) uV (×1,000)
0.25
1.25 280 nm 0.00 285 nm 240 nm
0.00
1.00 -0.25
0.75 -0.50 -0.25
-0.75
0.50 -0.50
-1.00
0.25 -0.75
-1.25 4
0.00
1 -1.50 6 -1.00 5
-0.25 7
-1.75 8
2 -1.25
-0.50 -2.00 9
-0.75 -2.25 -1.50
-2.50
-1.00 -1.75
5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5 min 12.0 12.5 13.0 13.5 14.0 14.5 15.0 15.5 16.0 16.5 17.0 17.5 min 12.0 12.5 13.0 13.5 14.0 14.5 15.0 min
uV (×1,000) uV (×1,000) uV (×1,000)
1.0 -0.75
270 nm -1.00 380 nm -0.5 350 nm
0.5 -1.25 -1.0
-1.50
0.0 -1.5
-1.75
-0.5
-2.00 -2.0
3 -2.25
-1.0
-2.5
-2.50 11
-1.5 -3.0 12
-2.75
-2.0 -3.00 -3.5
10
-3.25
-2.5 -4.0
-3.50
10.0 11.0 12.0 13.0 14.0 15.0 16.0 17.0 18.0 min 19.75 20.00 20.25 20.50 20.75 21.00 21.25 21.50 min 23.00 23.25 23.50 23.75 24.00 24.25 24.50 24.75 25.00 25.25 25.50 25.75 26.00 min
#FFG
uV (×1,000) uV (×1,000) uV (×1,000)
1.00 0.25
280 nm 0.00 285 nm 240 nm
0.75 0.00
-0.25
0.50 -0.25
-0.50
0.25 -0.75 -0.50
0.00 -1.00 -0.75
-1.25 4 -1.00
-0.25
1
-1.50 -1.25
-0.50 6 5
-1.75 -1.50 7
-0.75 2 8 9
-2.00 -1.75
-1.00
-2.25 -2.00
-1.25 -2.50
-2.25
5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5 min 12.0 12.5 13.0 13.5 14.0 14.5 15.0 15.5 16.0 16.5 17.0 17.5 min 12.00 12.25 12.50 12.75 13.00 13.25 13.50 13.75 14.00 14.25 14.50 14.75 15.00 min
uV (×1,000) uV (×1,000) uV (×1,000)
-0.75
270 nm 380 nm -0.5 350 nm
0.5 -1.00
-1.25 -1.0
0.0
-1.50
-0.5 -1.5
-1.75
-1.0 -2.00 -2.0
3 -2.25
-1.5 -2.5
-2.50 12
-2.0 11
-2.75 -3.0
-2.5 10 -3.00 -3.5
-3.25
-3.0
-3.50 -4.0
11.0 12.0 13.0 14.0 15.0 16.0 17.0 18.0 min 19.75 20.00 20.25 20.50 20.75 21.00 21.25 21.50 min 23.00 23.25 23.50 23.75 24.00 24.25 24.50 24.75 25.00 25.25 25.50 25.75 26.00 min
˙Peaks
1. Sulfadiazine, 2. Sulfamerazine, 3. Sulfadimidine, 4. Sulfamonomethoxine, 5. Trimethoprim, 6. Sulfamethoxazole, 7. Ormetoprim, 8. Sulfadimethoxine,
9. Sulfaquinoxaline, 10. Pyrimethamine, 11. Difurazon, 12. Nicarbazin (0.025 mg/L each)
Fig. 2 Chromatograms of Chicken and Beef:
Matrix Standard Solution (Red Line), Matrix Solution (Blue Line), Neat Standard Solution (Black Line)
First Edition: Oct. 2016
For Research Use Only. Not for use in diagnostic procedure.
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