Page 20 - Application Notebook - Solution for Food Safety

Application No.C136
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Repeatability Compounds were selected throughout the run at equi-
To assess the robustness of the system and the distant points (closest elution points to 3, 4, 5, 6, 7, 8,
developed method during routine analysis, repeat 9 and 10 minutes), including positive and negative ion
injections of a mint matrix sample spiked with 646 detection, (Table 3).
pesticides at 0.05 mg/kg, were analyzed over a 24 hour The peak area variance was less than 5.7 % for all
period. pesticides measured.
The results for selected compounds are displayed below
in Fig. 5.

Peak Area Response
Quantitation Ion
×10 6
4.5

4.0

3.5

3.0

2.5

2.0

1.5

1.0

0.5

0.0
0 10 20 30 40 50 60 70 80 90 100
Number of injections
Butocarboxim-sulfoxide Thiofanox-sulfone Monolinuron Probenazole
Dipropetryn Pyraflufen-ethyl Emamectin B1a Pyridalyl

Fig. 5 Peak area response for several pesticides following 100 repeat injections of a 0.05 mg/kg spiked into mint matrix extract.

Table 3 Peak area variance for selected following the repeated injection of a 0.05 mg/kg spiked into mint matrix extract
(number of sample replicates was 100; the analysis sequence was 24 hours).
MRM
CAS RT Average %RSD
Compound Name Formula M Polarity Quantitation
Number (mins) Peak Area (n=100)
Ion
Butocarboxim-sulfoxide 34681-24-8 C7H14N2O3S 206.0725 + 207.10 > 75.10 3.042 1,220,391 2.6
Thiofanox-sulfone 39184-59-3 C9H18N2O4S 250.0987 + 268.10 > 57.00 4.001 442,724 5.7
Monolinuron 1746-81-2 C9H11ClN2O2 214.0509 + 215.10 > 99.10 4.985 2,904,116 3.7
Probenazole 27605-76-1 C10H9NO3S 223.0303 + 224.00 > 41.05 5.995 1,145,189 3.5
Dipropetryn 4147-51-7 C11H21N5S 255.1518 + 256.20 > 144.05 6.999 3,289,597 3.4
Pyraflufen-ethyl 129630-19-9 C15H13Cl2F3N2O4 412.0204 + 413.00 > 339.00 8.004 3,653,333 3.5
Emamectin B1a 138511-97-4 C56H81NO15 1007.5606 + 886.40 > 158.20 9.008 3,109,562 4.5
Pyridalyl 179101-81-6 C18H14Cl4F3NO3 488.9680 - 491.90 > 109.05 10.171 1,579,422 5.0