Page 19 - Application Notebook - Solution for Food Safety

Application No.C136
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Final method performance for 646 pesticides Linearity
In order to test the performance of the developed Linearity was assessed over a six point calibration curve
method, linearity, repeatability and longer term from 0.002 - 0.1 mg/kg (2 - 100 pg/μL). All 646
robustness were assessed for all 646 pesticides. pesticides achieved excellent R values greater than
2
0.99 in both tomato and mint spiked extracts with
typical values greater than 0.996. Calibration curves
were generated using a linear curve fit type and 1/C
weighting. Typical calibration curve data is presented
below in Fig. 4.

Area Ratio 1.4 Dichlorprop | RT 4.379 min Area Ratio 4 Chloroxuron | RT 6.750 min
y = 0.014476x - 0.002314
y = 0.044471x -0.002057
R² = 0.9985819 R = 0.9992907
R² = 0.999649 R = 0.9998245
1.2
1.0 3
0.8 1.0e5 3.0e5
8.0e4 2.5e5
0.6 2 2.0e5
6.0e4
1.5e5
0.4 4.0e4
1 1.0e5
2.0e4
0.2 5.0e4
0.0e0 0.0e0
4.2 4.3 4.4 4.5 4.6 6.6 6.7 6.8 6.9
0.0 0
0 20 40 60 80 100 0 20 40 60 80 100
Conc. Ratio Conc. Ratio
Area Ratio 1.4 y = 0.014476x - 0.002314 Area Ratio 3.0 Novaluron | RT 7.734 min
Oxycarboxin | RT 4.433 min
y = 0.031448x + 0.003975
R² = 0.9985819 R = 0.9992907
R² = 0.9998738 R = 0.9999369
1.2
1.0 2.5
2.0
0.8 1.2e5 1.0e5
1.0e5 1.5 8.0e4
0.6 8.0e4
6.0e4
6.0e4 1.0
0.4 4.0e4
4.0e4
0.2 2.0e4 0.5 2.0e4
0.0e0 0.0e0
4.3 4.4 4.5 4.6
0.0 0.0 7.6 7.7 7.8 7.9
0 20 40 60 80 100 0 20 40 60 80 100
Conc. Ratio Conc. Ratio

Triazoxide | RT 8.909 min
Promecarb | RT 6.120 min
Area Ratio 17.5 y = 0.168487x - 0.008874 Area Ratio 7 6 y = 0.070322x - 0.003973
15.0
R² = 0.9995182 R = 0.9997591
R² = 0.999672 R = 0.999836
12.5
5
10.0
5.0e5 4
1.5e5
7.5 4.0e5 3
3.0e5 1.0e5
5.0 2
2.0e5
5.0e4
2.5 1.0e5 1
0.0e0 0.0e0
0.0 6.0 6.1 6.2 6.3 0 8.7 8.8 8.9 9.0 9.1
0 20 40 60 80 100 0 20 40 60 80 100
Conc. Ratio Conc. Ratio
Fig. 4 Calibration curves for selected pesticides spiked into a mint matrix extract in the range 0.002 - 0.1 mg/kg.
The quantitation MRM chromatogram is shown in black (qualifier ion MRM chromatograms are shown in
red and blue).