Page 22 - Application Notebook - PFAS Analysis

Application No. C184
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Table 8. Average conc. (n = 7), % recovery and % RSD of surrogates in spiked samples using LCMS-8045 and LCMS-8050

LCMS-8045 LCMS-8050
Fortified
Compound
Conc. (ng/L) Average Conc Average Conc
(ng/L), n=7 % Recovery % RSD (ng/L), n=7 % Recovery % RSD

M2PFHxA 40 43 107 14 40 101 10

M2PFDA 40 44 109 12 42 106 13

M-N-EtFOSAA 160 175 109 14 160 100 12

Surrogate Recovery  Summary and Conclusions
Surrogate recovery was similarly investigated by first This application note described and demonstrated the
spiking the water samples with surrogates, prior to use, performance and compatibility of Shimadzu
sample extraction. The spiked water samples undergo UFMS™ for EPA Method 537 with seven additional
the sample preparation procedures and LCMS PFAS targets. In this study, all samples and blanks
analysis. The calculated recovery of the surrogates were extracted by the same SPE procedure and
must be in the range of 70–130% (EPA, Section 9.3.5) analyzed using the same LC-MS/MS method on
to demonstrate good method performance. different instrument models. Method detection limits of

Seven water samples were spiked with 10 ng of 0.7–1.7 ng/L (LCMS-8050) and 0.7–3.3 ng/L (LCMS-
8045) were obtained and both systems fulfilled all of
M2PFHxA, 10 ng of M2PFDA and 40 ng of M-N- the EPA’s requirements for PFAS analysis in drinking
EtFOSAA giving a concentration of 40 ng/L for water. This was achieved with a 1 µL injection volume,
M2PFHxA and M2PFDA and 160 ng/L for M-N- in contrast to 10 µL as described in the original EPA
EtFOSAA in the 250 mL water sample. The calculated M537. The smaller injection volume (less burden on
recoveries obtained using LCMS-8045 and LCMS- the LCMS) would make the method more robust and
8050 are shown in Table 8 using a Mean Response reduce the long-term cost of ownership. Moreover,
Factor. All recoveries were within ± 10%, well Shimadzu’s high-speed and high-sensitivity UFMS™
achieving EPA requirements.
instruments allow users to further increase productivity
by running multiple EPA methods on the same system,
in which case using the LCMS-8050 is recommended.

 References

[1] U.S. EPA, "EPA Method 537: Determination of
Selected Perfluorinated Alkyl Acids in Drinking Water
UFMS, Nexera and Shim-pack are trademarks of Shimadzu
Corporation. by SPE and LC/MS/MS," Washington D.C., 2009.
ISOLUTE is a registered trademark of Biotage AB.
[2] ASTM International, "ASTM D7979-17: Standard
Test Method for Determination of Perfluorinated
Compounds in Water, Sludge, Influent, Effluent and
Wastewater by LC/MS/MS," Conshohocken PA, 2017.

First edition: January, 2019

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