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4-4. Chromatogram Assessment Results (Step 1)

            The assessment results for the chromatograms of Step 1 using   this investigation, the Kinetex XB-C18 Core-Shell column re-
            the quantitative assessment method of Section 4-3 are shown in   ceived the highest assessment among the columns when used in
            Fig. 8 (Upper tier: Shim-pack Series, Lower tier: Kinetex Series),   combination with a phosphate buffer solution (pH 2.6) and ace-
            and the results in the order of higher-assessed chromatograms   tonitrile as mobile phases. In addition, from the results of Fig. 8
            are shown in Fig. 9. Also, the higher-assessed combinations of   and Fig. 9, good correlation was clearly obtained between the
            separation columns and mobile phases are shown in Table 2. In   assessment values and the chromatogram's visual assessments.

                            450
                               Shim-pack XR series                         No.4
                            400
                                                                   No.5
                            350
                           Assessment Value  250
                               300
                            200
                            150
                            100
                            50
                             0





                            450
                               Kinetex series       No.2         No.3     No.1
                            400
                           Assessment Value    250
                            350
                            300
                            200
                            150
                            100
                            50
                             0




                                       Fig. 8  Chromatogram Quantitative Assessment Results (Step 1)


                                              No.1


                                                            Table 2  Chromatogram Quantitative Assessment Results (Step 1)
                                              No.2
                                                           Assessment  Separation
                                                            Value     Column      Mobile Phase A  Mobile Phase B
                                                           No.1 415  Kinetex XB-C18  Phosphate buffer solution (pH 2.6)  Acetonitrile
                                              No.3
                                                           No.2 407  Kinetex C18  Phosphate buffer solution (pH 2.6)  Acetonitrile
                                                           No.3 382  Kinetex XB-C18  Citrate buffer solution (pH 3.1)  Acetonitrile

                                              No.4
                                                           No.4 380  Sim-pack XR-C8  Phosphate buffer solution (pH 2.6)  Methanol
                                                           No.5 356  Sim-pack XR-C8  Citrate buffer solution (pH 3.1)  Acetonitrile
                                              No.5




             Fig. 9  Quantitative Assessment Higher Ranked Chromatograms
                       (Step 1)

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