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4-4. Chromatogram Assessment Results (Step 1)
The assessment results for the chromatograms of Step 1 using this investigation, the Kinetex XB-C18 Core-Shell column re-
the quantitative assessment method of Section 4-3 are shown in ceived the highest assessment among the columns when used in
Fig. 8 (Upper tier: Shim-pack Series, Lower tier: Kinetex Series), combination with a phosphate buffer solution (pH 2.6) and ace-
and the results in the order of higher-assessed chromatograms tonitrile as mobile phases. In addition, from the results of Fig. 8
are shown in Fig. 9. Also, the higher-assessed combinations of and Fig. 9, good correlation was clearly obtained between the
separation columns and mobile phases are shown in Table 2. In assessment values and the chromatogram's visual assessments.
450
Shim-pack XR series No.4
400
No.5
350
Assessment Value 250
300
200
150
100
50
0
450
Kinetex series No.2 No.3 No.1
400
Assessment Value 250
350
300
200
150
100
50
0
Fig. 8 Chromatogram Quantitative Assessment Results (Step 1)
No.1
Table 2 Chromatogram Quantitative Assessment Results (Step 1)
No.2
Assessment Separation
Value Column Mobile Phase A Mobile Phase B
No.1 415 Kinetex XB-C18 Phosphate buffer solution (pH 2.6) Acetonitrile
No.3
No.2 407 Kinetex C18 Phosphate buffer solution (pH 2.6) Acetonitrile
No.3 382 Kinetex XB-C18 Citrate buffer solution (pH 3.1) Acetonitrile
No.4
No.4 380 Sim-pack XR-C8 Phosphate buffer solution (pH 2.6) Methanol
No.5 356 Sim-pack XR-C8 Citrate buffer solution (pH 3.1) Acetonitrile
No.5
Fig. 9 Quantitative Assessment Higher Ranked Chromatograms
(Step 1)
4