Page 6 - LC-SFC_Pharma_Brochure

3. Method Scouting Solution 4. Building Analytical Conditions for
Simultaneous Analysis of Cephem
Fig. 3 shows a screen capture from Method Scouting Solution, the
Antibiotics
special software used with the Nexera Method Scouting System.
Method Scouting Solution is a software application specially Here we introduce an application example using the Nexera
designed to support automation of the method scouting process, Method Scouting System. Thirteen types of Cephem antibiotics
while providing a user-friendly graphical interface to facilitate the were used as analytes, including cephradine, cephalexin, cephapirin,
scouting setup operations. Settings for the columns, mobile cefazolin, cefadroxil, cefaclor, cefuroxime, cephalothin, cefotaxime,
phases, and gradient conditions are made in the main window cefoperazone, cefoxitin, cefsulodin and cefmetazole. Cephem
(Fig. 3), and by merely entering the necessary parameters accord- antibiotics are a type of β-lactam antibiotic, and because they are
ing to the provided on-screen guidance, setup can be easily broad-spectrum antibiotics possessing strong antibacterial activity,
completed through to creation of the batch table. With the built- they are commonly used as injectable and oral medicines. However,
in database management functions, entering and storing the since these compounds possess similar structures, their complete
names of columns and mobile phases not only improves manage- separation by reversed-phase chromatography is notoriously
ment efficiency, it can help to ensure much fewer operational difficult. Fig. 5 shows the workflow used for building the analytical
mistakes, especially when multiple operators share the system. conditions for simultaneous analysis of the cephem antibiotics.
Furthermore, since Method Scouting Solution integrates seamlessly
NH2 NH2
H HH H HH N H HH
with Shimadzu's LabSolutions analysis software, a series of analysis N S N S S N S
O N O N O N O CH3
schedules for method scouting, from analysis preparation at O CH3 O CH3 O O
O OH O OH O OH
instrument startup to analysis completion, can be created

cephradine cephalexin cephapirin
automatically. This achieves a significant reduction in the time and
NH2 NH2
H HH H H H H H H
effort spent on setting method scouting conditions and creating N N S N S N S
N N O N S S CH3 O
the batch table. For details regarding Method Scouting Solution, N O HO O O N CH3 O N Cl
NN
O OH
also refer to the Technical Report "Ultra Fast Method Scouting." O OH O OH
cefazolin cefadroxil cefaclor
O
N CH3 H H H O CH3
H H H N S S N H H H
N S N S
S H2N
O O N O CH3 N
O N O NH3 O O N O CH3
O O O
O O OH O
O OH O OH
cefuroxime cephalothin cefotaxime
OH
H3C
H OH
O O S
H HH N
O N S
N N CH3 S O N O NH2
H3C N O N S N O
O N O
NN O O H
O OH
cefoperazone cefoxitin
O H3C
O
O S O O H N O H S
H H H CH3
N S HO S
NH2
NH2 O N S N
O N N O N
O
N N
O O
O OH
cefsulodin cefmetazole
Fig. 4 Cephem Antibiotic Substances
Workflow
Step 1ɹScouting for mobile phase and column
<Evaluation 1> Chromatogram pattern confirmation
(number of detected peaks, resolution)
Step 2ɹOptimization of gradient conditions
<Evaluation 2> Chromatogram pattern confirmation
(number of detected peaks, resolution)
Establish simultaneous analysis conditions
Fig. 3 Method Scouting Solution Fig. 5 Method Scouting Workflow
2