Page 53 - LC-SFC_Pharma_Brochure

5. Chiral Separation
5. Chiral Separation
In the field of pharmaceuticals, research is underway in the area of drug
discovery using chiral columns for rapid chiral separation. Finding the
appropriate combination of analytical column and mobile phase for a
given analyte from the wide variety of chiral columns available requires
a substantial amount of time and labor. Therefore, there is a demand
to improve the speed of condition scouting for chiral separations.
The speed and labor required for scouting chiral compound separation
conditions can be improved by combining Shimadzu's Nexera UC chiral
screening system and the wide range of polysaccharide derivative
CHIRALPAK and CHIRALCEL series chiral columns (Daicel Corporation).
The Nexera UC chiral screening system includes an SFC system, solvent
switching valves, and column switching valves and is able to acquire
comprehensive data by automatic and continuous screening of the Fig. 11 Configuration Window of Method Scouting Solution Software
modifier conditions on a maximum of 12 columns. Its mobile phase
blending function can also mix up to four different solvents to user-de- Column Modi er
fined ratios for analysis under a variety of separation conditions, which (1) CHIRALPAK IA-3/SFC (1) MeOH
significantly simplifies the workflow of condition scouting for chiral (2) CHIRALPAK IB-3/SFC (2) EtOH
compounds. (3) CHIRALPAK IC-3/SFC (3) Acetonitrile/EtOH 75/25 (V/V)
(4) CHIRALPAK ID-3/SFC
Also, Method Scouting Solution for Nexera UC is software that pres- (5) CHIRALPAK IE-3/SFC Modiÿer conc : 20 %
Flow rate
: 3.0 mL/min
ents a graphical user interface environment developed to support the (6) CHIRALPAK IF-3/SFC Temperature : 40°C
: 10 MPa
Back pressure
process of separation condition scouting for chiral compounds (Fig. (7) CHIRALPAK AD-3/SFC Injection volume : 1 µL
11). This software provides database management for analytical col- (8) CHIRALPAK AS-3/SFC Detection : UV (300 nm)
(9) CHIRALPAK AY-3/SFC
umns, mobile phases, and modifiers, which improves management ef- (10) CHIRALCEL OD-3/SFC
ficiency and can reduce the number of operating errors that arise with (11) CHIRALCEL OJ-3/SFC
multiple operators. The software provides powerful support for work (12) CHIRALCEL OZ-3/SFC
related to chiral compound analysis, including work such as the calcu- 3.0 mm I.D. × 100 mm L. 3 µm
lation of required modifier and sample volumes, column washing,
changeover of enclosed liquids at Fig. 12 Screening Conditions
the end of analysis to prevent
column degradation, and estima- Modi er: MeOH Modi er: ACN/EtOH = 75/25 (v/v)
tion of analysis completion times. IA IB IC ID IA IB IC ID
Here, we present chiral separation
screening results for omeprazole IE IF AD AS IE IF AD AS
obtained from all 36 possible com-
binations of the 12 chiral columns
(Daicel Corporation) and three AY OD OJ OZ AY OD OJ OZ
modifier conditions (Fig. 12, 13).
The Nexera UC chiral screening Modi er: EtOH
system utilizes SFC to select the mAU
IA IB IC ID
mobile phase and optimize the CHIRALCEL OZ-3/SFC
MeOH
separation conditions in a short 600
time period, which improves R&D IE IF AD AS 400 Resolution: 7.97
efficiency during the drug discov-
ery stage of pharmaceutical pro- 200
duction. AY OD OJ OZ
0
0.0 1.0 2.0 3.0 4.0 min
Fig. 13 Screening Results
CHIRALPAK and CHIRALCEL are registered trademarks of Daicel Corporation.

First Edition: September, 2016
For Research Use Only. Not for use in diagnostic procedures.
This publication may contain references to products that are not available in your country. Please contact us to check the availability of these
products in your country.
The content of this publication shall not be reproduced, altered or sold for any commercial purpose without the written approval of Shimadzu.
Company names, products/service names and logos used in this publication are trademarks and trade names of Shimadzu Corporation, its
subsidiaries or its afÿliates, whether or not they are used with trademark symbol “TM” or “®”.
Third-party trademarks and trade names may be used in this publication to refer to either the entities or their products/services, whether or not
they are used with trademark symbol “TM” or “®”.
www.shimadzu.com/an/ Shimadzu disclaims any proprietary interest in trademarks and trade names other than its own.
The information contained herein is provided to you "as is" without warranty of any kind including without limitation warranties as to its
accuracy or completeness. Shimadzu does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the
use of this publication. This publication is based upon the information available to Shimadzu on or before the date of publication, and subject
to change without notice.
© Shimadzu Corporation, 2016
Printed in Japan