Page 58 - LC-SFC_Pharma_Brochure

5-4. Sample Solution Analysis Examples

Examples of analysis of the sample solutions are shown in Fig. 8−10. cal conditions were the same as those used for analysis of the
All of these were analyzed using a 1/10 dilution of the sample stock standard solutions.
solutions prepared using the automatic dilution feature. The analyti-

uV uV uV
˙Peak ˙Peak ˙Peak
40000 1. Caffeine 40000 1. Caffeine 40000 1. Caffeine
1
30000 30000 30000
20000 20000 20000
1
10000 10000 10000 1
0 0 0
0.0 2.5 min 0.0 2.5 min 0.0 2.5 min
Fig. 8 Chromatogram of Coffee Fig. 9 Chromatogram of Black Tea Fig. 10 Chromatogram of Green Tea

6. Overlap Injection Feature
Using the new overlap injection feature of LabSolutions makes it this analysis, where the pretreatment took 6 minutes and analysis
possible to start pretreatment operations for the next analysis while took 7 minutes, utilizing the overlap injection for preparation of the
the current analysis is in progress. This permits shortening of the 3 solutions allowed shortening of the analysis time by 31% compared
analysis time of the subsequent analysis by one cycle compared to a to the standard analysis without overlapping injections (Fig. 11).
typical analysis if the analysis is started after the pretreatment (*3) . In
*3 Note that the overlap injection cannot be implemented if the autosampler is set for "internal and external needle rinse".

Without Overlap Injection Pretreatment (6 min) Analysis (7 min) Pretreatment (6 min) Analysis (7 min) Pretreatment (6 min) Analysis (7 min)

Pretreatment (6 min) Analysis (7 min)
Analysis time shortened by about 31 %
With Overlap Injection Pretreatment (6 min) Analysis (7 min) Pretreatment (6 min) Analysis (7 min)
27 min 39 min
Fig. 11 Shortened Analysis Time Using Overlap Injection

7. Summary

Pretreatment operations were shown to require less time and effort
when using the automatic dilution feature of the SIL-30AC. Furthermore,
these automated functions allow highly accurate results, free of
technician-related errors.
When using the SIL-30AC, automatic dilution and automatic reagent
addition can easily be set up by using standard templates. Also, use of
pretreatment programs makes it possible to set up very complex
operations for a wide range of functions to be executed automatically.
Analyses that are conducted utilizing the autosampler pretreatment features introduced here do not employ a calibrated volumenometer, and are therefore unsuitable if traceability is required.

First Edition: July, 2012

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