Page 60 - LC-SFC_Pharma_Brochure

i-DReC

Area (×10 )
6
Unique quantitative data processing function
30 Correlation factor
• When the peak intensity of PDA detector data exceeds the R=0.9996726
user-defined threshold value, i-DReC automatically shifts 25
the chromatographic profile to a wavelength with less UV
absorption to prevent signal saturation, so as to avoid
wrong quantitative results caused by the chromatographic 20 Correction
quantitative peak exceeding the upper limit of the detector. by i-DReC
15
• The absorption ratio between the original target wavelength
and the wavelength used by the i-DReC function is applied
as a correction factor to the peak area of the acquired 10 Original
chromatogram, thereby calculating the peak area and
height at the original target wavelength. 5

Accurately quantify high concentration sample
0
0 0.25 0.5 0.75 1
• Faced with high concentration target products in the Conc. (g/L)
monitoring of synthetic reaction processes, a broader linear Peak area correction and linear
range of standard curves can be established, making it dynamic range extension
easier to quantify accurately at all concentrations.
mAU 250 nm, 4 nm
• Accurate quantitative results can be obtained with one 100
injection without additional dilution when analyzing the
content of active ingredients and impurities in the drug. 75 Main
The efficiency of laboratory analysis can be significantly
improved.
50 Imp3
Imp2
Extraction of chromatogram at λb 25
mAU Imp1
4000 λa nm 0
3.5 4.0 4.5 5.0 5.5 6.0
min
Threshold mAU
level 20
λb nm Main
10 Imp1 Imp2 Imp3
0

Extraction of 4.0 4.1 4.2 4.3 4.4 4.5 4.6
spectrum for min
sensitivity correction
mAU
Signal intensity for Retention Mean Area Area Area
extraction of sensitiity Absorption ratio Peak Time (min) (µAUsec) %RSD Ratio (%)
correction spectrum is calculated by Ia
and Ib 1 (Main) 4.634 31,123,746 0.06 --
Sensitivity k = Ia 2 3 5.448 925,522 0.12 2.974

64,161
0.08
0.206
3.900
spectrum
Ia correction Ib 4 4.910 32,810 0.15 0.105
5 5.091 15,103 0.16 0.049
6 4.487 9,487 0.26 0.030
λa 7 4.226 7,981 0.28 0.026
8 4.975 7,981 0.44 0.026
Ib λb
Imp1 4.056 2,001 0.27 0.006
λ Imp2 4.331 2,440 0.85 0.008
Imp3 4.376 1,663 0.65 0.005
i-DReC correction provided peak area
Basic principle of i-DReC reproducibility of 0.06% RSD for the saturated Peak1