Page 49 - LC-SFC_Pharma_Brochure

Solution 6: Chiral Separation

Among all the drugs used in the world, chiral drugs account for more than half. These chiral compounds (enantiomers)
may exhibit different responses to pharmacological activities and metabolic processes in the human body; some may show
therapeutic effects, or be deemed ineffective or in the worst case even harm the body. Accurate analysis of chiral impurities
that may occur during drug synthesis is an important part of ensuring drug safety.

When traditional normal phase or reversed phase chromatography is used for chiral separation, difficult column selection,
poor separation, long analysis time, and large solvent consumption are common problems.

SFC is widely used for the separation and analysis of chiral compounds. Shimadzu Nexera UC offers a variety of SFC
configurations to meet your different analytical testing needs in chiral separations.

Nexera UC SFC System

Chiral Screening System

HPLC Rs = 1.328

0 4 8 12 m n i
Improved ~3x runtime
resolution reduction

SFC Rs = 1.703

 Effective GUI for Chiral Screening System
0 1 2 3 4 m n i
Comparison of retention time and separation acquired by Conventional LC and SFC
(sample: α-tocopherol, column: Shim-pack UC-X Sil)

Ideal solution for chiral analysis
• The supercritical fluid with large diffusion coefficient and low
viscosity is used as the mobile phase. The modifiers, such
as methanol, with different polarities and proportions can
meet the separation and detection requirements of chiral
compounds with different polarities in a wider range.

• With any combination of columns and modifiers, a large
number of analytical methods are automatically generated
for screening, perfectly supporting the development of chiral
compound methods.

Perfect combination to increase efficiency and reduce cost
• Compared to traditional normal phase and reversed phase
chromatography, Nexera UC provides better separation
selectivity and faster analysis for difficult-to-separate isomers. Nexera UC SFC-UV System

• Nexera UC can help you to minimize organic solvent
consumption, and reduce analysis costs while reducing
environmental impact.