Page 32 - LC-SFC_Pharma_Brochure

Solution 3: Sample Preparation and Purification

The preparation and purification of drugs is an indispensable part of the pharmaceutical industry. In the process of drug
synthesis, both principal components and impurities need to be prepared to obtain sufficient amounts of a single compound
for research and other purposes.
The purity, speed and cost of preparation are important factors that pharmaceutical companies need to consider when
conducting separation and preparation.

Shimadzu offers flexible and comprehensive preparation and purification system solutions to meet the individual needs of
different users.

Preparative HPLC System

Analytical/Preparative System Recycle Preparative System Preparative LCMS System Analytical/Preparative System Recycle Preparative System Preparative LCMS System

Analytical PDA MS Analytical PDA MS
Fractionate Fractionate
Pump Detector Pump Separation Detector Pump Separation Pump Detector Pump Separation Detector Pump Separation
Fractionate Fractionate
Preparative Fractionate UV Preparative Fractionate UV
Recycle Recycle

non-target compound non-target compound
mV mV
6 1 Cycle Effective separation even with 6 1 Cycle Effective separation even with
st
st
A) Analytical Conventional UV Model: A) Analytical Conventional UV Model:
800 20x the injection volume 800 20x the injection volume
7 8 Many non-target compounds are 7 8 Many non-target compounds are
1 1
nd
nd
700 2 4 2 Cycle included in the target eluent 700 2 4 2 Cycle included in the target eluent
rd
rd
5 3 Cycle when using UV only 5 3 Cycle when using UV only
3 3
600 600
500 500
UV Chromatogram UV Chromatogram
400 400
300 B) Preparative 300 B) Preparative
MS Guide Model: MS Guide Model:
200 When using UV + MS, target 200 When using UV + MS, target
compounds are selectively compounds are selectively
100 100
fractionated fractionated
0 0
0 10 20 30 40 50 60 0 10 20 30 40 50 60
0 2 4 6 8 10 12 14 min 0 2 4 6 8 10 12 14 min
MS Chromatogram MS Chromatogram
Example of scale-up with the same column resins Example of scale-up with the same column resins
Customize prep-LC system for your dedicated needs
Analytical/Preparative System
• Based on the expandable Prominence series, Shimadzu’s Recycle Preparative System Preparative LCMS System
preparative LC system covers a wide range of configurations
from analytical to preparative, making it easy to prepare PDA MS
Analytical
different grades of products. Fractionate
Pump Detector Pump Separation Detector Pump Separation
• analytical/ Fractionate
Preparative UV
Fractionate

greatly improve laboratory productivity while satisfying more Recycle
uses.
non-target compound
mV
Achieve higher preparative efficiency Effective separation even with
st
A) Analytical 6 1 Cycle Conventional UV Model:
800 20x the injection volume
7 8 Many non-target compounds are
1 4 nd included in the target eluent
700
• These versatile configurations and freely selected detector 2 Cycle rd when using UV only
2
3 5 3 Cycle
600
types ensure that you don’t miss any impurities.
500
UV Chromatogram
• Analytical/preparative system makes the preparation scale-up
400
more convenient by supporting efficient transfer of analytical MS Guide Model:
300
B) Preparative
condition to preparative condition with the dual-flow system. When using UV + MS, target
200
compounds are selectively
100
fractionated
0
• Through the recycle preparative system, the column length 0 10 20 30 40 50 60
14
12
0
6
2
4
8
10
and column capacity can be expanded, and the resolution can min MS Chromatogram
Example of scale-up with the same column resins
be greatly improved under limited conditions, while avoiding a
large consumption of mobile phase