Page 31 - LC-SFC_Pharma_Brochure

4. i-Series Integrated Liquid
4-2.
ACTO Function
4. i-Series Integrated Liquid 4-2. ACTO Function
Chromatograph System and the
Chromatograph System and the ACTO, which is equipped in the latest version of LabSolutions, is an effi-
ACTO Function cient method transfer tool provided by Shimadzu. Here we describe one
ACTO Function
of ACTO's functions called "gradient start time adjustment function."
This report has described examples of retention time differences
caused by different system volume and adjustment of the initial hold Transferring an analytical method from an existing LC system to another
time (adjusting the gradient start time). We now describe the Shi- system can cause differences in retention times because of the differenc-
madzu i-Series integrated liquid chromatograph system and the es in system volume and specifications of solvent delivery unit. This prob-
ACTO function equipped in the Shimadzu LabSolutions workstation lem can be resolved using ACTO's gradient start time adjustment func-
software that supports a variety of method transfers. tion. The gradient adjustment function is configured during method cre-
ation. If a user simply enters the difference in system volume, then the
corrected initial hold time is automatically added or subtracted during
4-1. i-Series Integrated Liquid analysis. This enables the acquisition of identical chromatograms before
4-1. i-Series Integrated Liquid
omatograph System
Chromatograph System and after method transfer. The function can also correct subtle errors that
Chr
cannot be considered by the compatibility kit (e.g., pump characteristics
The i-Series is Shimadzu's product line of integrated liquid chromato-
graphs that contain all the functions required for LC analysis in a and solvent delivery mechanism) and can achieve optimal compatibility.
compact unit. These functions have been optimized for ease of oper- This adjustment is configured in a method separately from the time pro-
ation and maintenance. Using standard piping or attaching the op- gram. Thus, reconfiguration of an existing time program is unnecessary.
tional compatibility kit enables the use of i-Series systems with system Consequently, using Shimadzu's i-Series instruments and the ACTO
volumes compatible with other Shimadzu systems and other vendors' function can provide higher efficiency and reliability during method
systems. This provides good reproducibility between systems when transfer in a variety of applications.
performing analyses using existing methods.
Original instrument used before method transfer
Shimadzu's workstation software also includes the ACTO function, as The mobile phase composition begins to change once this
mentioned earlier, which is designed specifically for the i-Series and period of time has elapsed after sample injection.
enables smooth method transfer.

System volume
(large) The point where the mobile phase
composition begins to change.
Instrument used after method transfer

Without ACTO

The gradient starts earlier than that
from the original instrument due to
System volume the difference of system volume.
(small)
With ACTO

Fig. 6 i-Series Integrated LC System (Nexera-i) An initial holding time equivalent to
the speci ed system volume is added
to the gradient start time automatically.
Fig. 7 Adjusting the Gradient Start Time

First Edition: August, 2016
For Research Use Only. Not for use in diagnostic procedure.
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