Page 27 - LC-SFC_Pharma_Brochure

4. Nexera-i MT and ACTO Function
4. Nexera-i MT and ACTO Function However, transferring a method requires complex calculations based on
the internal diameter of the column, column length, particle diameter,
We describe the Shimadzu Nexera-i MT integrated liquid chromatograph and other parameters for determining an appropriate post-transfer flow
system and the ACTO function included in the Shimadzu LabSolutions rate and time program. This task can be simplified by using the "Method
workstation software, which are products that support method transfer. Transfer" tool included in LabSolutions. When this tool is used, a method
is created automatically by just selecting an existing method, and enter-
4-1. Nexera-i MT Integrated Liquid
4-1. Nexera-i MT Integrated Liquid ing the column information and some other parameters. Automatically
Chromatography System
Chr omatography System created methods can be incorporated in LabSolutions software with a
single click of a button; this streamlines the work flow from method con-
version to analysis. Method Transfer is a powerful tool and can be uti-
Nexera-i MT is an excellent method transfer system from existing LC
systems. Nexera-i MT has dual flow lines, one is for HPLC and the lized in a variety of situations to carry out efficient method transfers.
other for UHPLC, and can switch these flow lines during a method.
This system offers following two advantages:
• The ability to switch HPLC flow line and UHPLC flow line enables the
transfer of UHPLC methods to general use (HPLC) methods and to trans-
fer general use methods to UHPLC methods within a single system.
• Attaching an optional flow line to the standard HPLC flow line
allows for method compatibility with liquid chromatography (LC)
systems that have different system volumes. Step 1: Tool Startup
Combining the Nexera-i system with the ACTO function described
below facilitates smooth method transfer in a variety of applications.

Step 2: Method File Selection

Fig. 4 Nexera-i MT

4-2. ACTO Function (Method Transfer)
4-2. ACTO Function (Method T ransfer)
ACTO, which is included in the latest version of LabSolutions, is an ef- Step 3: Column Speci cation
ficient method transfer tool provided by Shimadzu. Here we describe
one of ACTO's functions called "Method Transfer."
For improving the speed of an existing analysis performed on a HPLC
system by converting it for use on a UHPLC system or for converting an
analysis performed on an UHPLC system for general use with HPLC, Step 4: Save as Method File
modifications need to be made to the existing analysis methods. Fig. 5 Method Transfer Work Flow

First Edition: August, 2016
For Research Use Only. Not for use in diagnostic procedure.
This publication may contain references to products that are not available in your country. Please contact us to check the availability of these
products in your country.

The content of this publication shall not be reproduced, altered or sold for any commercial purpose without the written approval of Shimadzu.
Company names, product/service names and logos used in this publication are trademarks and trade names of Shimadzu Corporation or its
afÿliates, whether or not they are used with trademark symbol “TM” or “®”. Third-party trademarks and trade names may be used in this
publication to refer to either the entities or their products/services. Shimadzu disclaims any proprietary interest in trademarks and trade names
www.shimadzu.com/an/ other than its own.
The information contained herein is provided to you "as is" without warranty of any kind including without limitation warranties as to its
accuracy or completeness. Shimadzu does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the
use of this publication. This publication is based upon the information available to Shimadzu on or before the date of publication, and subject
to change without notice.
© Shimadzu Corporation, 2016
Printed in Japan