Page 23 - LC-SFC_Pharma_Brochure

Solution 2: LC Method Transfer

When the R&D department completes the development and verification of the LC method, it needs to be transferred to
other laboratories such as QA/QC and CMO.

Due to the use of different analytical instruments and configurations, pharmaceutical companies may encounter difficulties
with LC method transfer in their drug development and research processes. Issues such as irreproducibility of resolution
and retention time may be faced.

Shimadzu understands this problem and developed the Nexera-iMT method transfer system. Its Analytical Condition
Transfer and Optimization (ACTO) function achieves seamless transfer of LC analytical methods.

Nexera-iMT

i) Method transfer between ii) Method transfer between systems with different delay volume
HPLC and UHPLC

Original instrument used before method transfer
Prominence-i The mobile phase composition begins to change once this System 1: System volume 1155 µL
period of time has elapsed after sample injection.

System volume
(large) The point where the mobile phase
composition begins to change.
System 2: System volume 505 µL
Instrument used after method transfer
0.0 10.0 20.0 30.0 40.0 min
Without ACTO
Method Speed
Transfer Improvement
The gradient starts earlier than that
from the original instrument due to
Nexera-i System volume the difference of system volume. System 2 + Added initial hold (+650 µL)
(small)
With ACTO
An initial holding time equivalent to
to the gradient start time automatically.
0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 min
0.0 2.0 4.0 6.0 8.0 10.0 12.0 min

Method transfer of Cephem antibiotics Retention time correction by adding an initial holding time

Excellent dual-flow method transfer system
• The new method transfer system based on the outstanding
i-Series ensures excellent intersystem reproducibility. Unique
dual-flow system is designed for both HPLC and UHPLC
analysis and transfer.

• The new ACTO function can automatically adjust the delay
volume to fit different systems. Within a few clicks, the new
Nexera-iMT method can be generated.

Transfer methods easily

• Using Nexera-iMT, analytical methods can be freely converted
to fit HPLC and UHPLC systems. This greatly simplifies the
method transfer process and maximize laboratory efficiency.

• The chromatograms of the original method were perfectly
reproduced, and the separation effect and retention time of
the original condition were maintained, regardless of system Nexera-iMT
differences such as instrument model and delay volume.