Page 16 - LC-SFC_Pharma_Brochure

2. Simultaneous Analysis of Sulfa 2-1. Method Scouting Solution
Drugs Using Method Scouting The main window displayed in Method Scouting Solution is shown in
Solution Figure 4. It shows the various parameters for columns, mobile phases,

This section describes an example of scouting conditions for simul- sample information, gradient conditions, and so on, and corre-
taneous analysis of sulfa drugs, which are used in synthetic antibac- sponding icons that can be clicked to easily change the respective
terial agents, anticancer agents, and other pharmaceuticals. The ini- parameters.
tial conditions considered for the sulfa drugs are shown in Figure 3. Using the dedicated software, batch analysis can be performed
In this case, a total of four types of mobile phases were considered (scouting started) easily by (1)“selecting the columns and mobile phases
by combining either of two types of (sodium) phosphate buffer solu- preregistered in the database, (2)“specifying gradient conditions, (3)“
tions with different pH values as solvent“A and acetonitrile or metha- entering sample information and other parameters, and (4)“clicking the
nol as solvent“B. Six types of columns were considered, including [Create Batch] button. By working in conjunction with LabSolutions
three Shim-pack XR“series columns. These four mobile phases and six software, the Method Scouting Solution software is able to automate
columns were used to test a total of 24“analytical condition combi- all analysis processes involved in method scouting, from preparing the
nations. system for analysis (startup) to shutting down the system after the
analysis is ÿnished (shutdown). Consequently, the software can sig-
niÿcantly reduce the amount of time and effort required to specify
conditions and execute analyses for method scouting.

Analytes: Nine Sulfa Drug Compounds

H2N H2N
O H2N O H2N H2N
S O N S O N N O O N N
O NH S N N O NH S S N O
N N O H N N O N H O H
1. Sulfadiazine 2. Sulfamerazine 3. Sulfadimidine 4. Sulfamethoxypyridazine 5. Sulfamonomethoxine

H2N
H2N H2N Na O
O H2N O S
S O O N S O NH
O NH S O NH N
N O N H N N
O
O N O
6. Sulfamethoxazole 7. Sulfisoxazole 8. Sulfadimethoxine 9. Sulfaquinoxaline sodium

Mobile Phases: 2 Aqueous × 2 Organic = 4 Mobile Phases Columns: 6 Types
(A) (a) 10–mmol/L (sodium) phosphate buffer, pH 2.6 1. Shim-pack XR-ODS (75–mm–L. × 3.0–mm–I.D., 2.2–µm)
(b) 10 mmol/L (sodium) phosphate buffer, pH 6.9 2. Shim-pack XR-C8 (75–mm–L. × 3.0–mm–I.D., 2.2–µm)
3. Shim-pack XR-Phenyl (75 mm L. × 3.0 mm I.D., 2.2 µm)
(B) (a) Acetonitrile 4. Column A-ODS (75 mm L. × 3.0 mm I.D., 2.0 µm)
(b) Methanol 5. Column B-C8 (75 mm L. × 3.0 mm I.D., 2.0 µm)
6. Column C-Phenyl (75 mm L. × 3.0 mm I.D., 2.0 µm)
4 types of 6 types of
mobile phases columns Total 24 combinations

Other Analytical Conditions
Flow rate : 0.8 mL/min
Injection volume : 2 µL
Column temperature : 45 ºC
Detection : UV detection (265–nm)
Time program : B Conc. 10–% (0–min) ĺ 35–% (6.50–min) ĺ 50–% (6.51 to 7.50–min) ĺ 10–% (7.51 to 11.00–min)

Fig. 3 Scouting Analytical Conditions for Simultaneous Analysis of Sulfa Drugs

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