Page 14 - LC-SFC_Pharma_Brochure

4. Analysis
4. Analysis
CLASS-Agent Report data processing software (Shimadzu Corp.) can number of detected peaks, etc. With this software, it is possible to
quickly select the best separation conditions by comparing the data compare data both visually and quantitatively, thus making data pro-
trace along with chromatographic parameters such as resolution, cessing more efficient.

Resolution Separation Factor Symmetry Factor (Peak 1) Symmetry Factor (Peak 2)
k (Peak 1) k (Peak 2) Area % × 10 (Peak 1) Area % × 10 (Peak 2)
10
9
8
7
6
5
4
3
2
1
0
1 2 3 4 5
Order
Fig. 6 Quantitative Comparison of Chromatograms Using CLASS-Agent Report

Table 2 Analysis Results for Methylclothiazide
Separation Symmetry Factor k Area % No. of Peaks
Order Run No. Analytical Conditions Resolution
Factor Peak 1 Peak 2 Peak 1 Peak 2 Peak 1 Peak 2 Detected
1 18 Methylclothiazide_ID_n-Hex_EtOH_3_analysis_B20%_14 min 3.785 1.523 1.31 1.463 5.665 8.626 49.777 50.223 2
2 27 Methylclothiazide_IF_MC_EtOH_6_analysis_B2%_4 min 3.086 1.858 1.127 1.094 1.39 2.583 52.748 47.252 2
3 6 Methylclothiazide_IB_MC_EtOH_6_analysis_B2%_4 min 2.456 2.248 0.715 1.094 0.443 0.995 45.633 54.367 2
4 13 Methylclothiazide_IC_n-Hex_EtOH_3_analysis_B20%_14 min 1.577 1.238 1.264 1.3 2.821 3.493 47.96 52.04 2
5 30 Methylclothiazide_IF_n-Hex_EtOH_4_analysis_B100%_18 min 1.515 2.759 1.465 0 0.102 0.282 48.153 51.847 2

5. Conclusion
5. Conclusion
as well as a visual comparison, permitting greatly improved efficiency
Using the Nexera Method Scouting system and the iChiral-6 high-res-
of data analysis.
olution polysaccharide-based columns, it was possible to quickly de-
termine through exhaustive analysis the best column and mobile We believe that the system and columns used in this research will be
phase for each of three types of chiral compounds. Further, by com- useful in a variety of markets including the synthetic drug discovery
paring such numerical values representing the resolution and symme- sector and pharmaceutical intermediates sector responsible for chiral
try index of each chromatogram using CLASS-Agent Report, it was analysis, new method development in pharmaceutical CMC depart-
possible to conduct a comparative evaluation of the chromatograms ments, and chemical and food R&D.

References
1) Technical Report “Ultra Fast Method Scouting” (C190-E158)
2) Technical Report “Improved R&D Efficiency Through Speedier Method Development (3)” (C190-E159)
Acknowledgment
We wish to express our deep appreciation to Daicel Corporation for their guidance and cooperation for us to proceed with this study.
Note: CHIRALPAK is a registered trademark of Daicel Corporation.

First Edition: November, 2013

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